2', 7'-Dichlorofluorescein di-β-D-galactopyranoside (DCFDG)

Product ID: M1194



Unit SizePriceQuantity 
25mg
$103.08
  • Buy 5 for $82.47 each and save 20%

Availability: In stock


Description

Highly sensitive fluorescent substrate for measuring galactosidase and galactocerebrosidase activity inside of live cells and lysosomes.

This substrate releases the highly fluorescent fluorophore 2',7'-dichlorofluorescein (EX: 495nm/EM: 529 nm) at the site of galactosidase or galactocerebrosidase activity. Since the pKa of the released fluorophore is significantly lower than comparable fluorophores, it can retain appreciably more fluorescence in the highly acidic environment of the lysosome than other similar fluorophores. Note: Absorption prior to enzyme hydrolysis is 290 nm; 4.7K).

Technical Data
SKU M1194
Unit Size 25mg
Alternative Names Dichlorofluorescein di-Galactoside, DCFDG
Absorption 495nm
Extinction 529
Detection Method Fluorescence
Molecular Formula C₃₂H₃₀Cl₂O₁₅
Molecular Weight 725.48
Soluble In DMSO, DMF, sl. H2O
Storage Conditions -20C, Desiccated, Protect From Light
Notes High Purity Grade (>99%). Absorption and Emission were measured when product released fluorophore

References and Citations

References:

  • van Es H.H., Veldwijk M., Havenga M., Valerio D. (1997) "A flow cytometric assay for lysosomal glucocerebrosidase" Anal. Biochem. 247: 268-271.
  • Chan KW., Waire J., Simons B., (2004) "Measurement of lysosomal glucocerebrosidase activity in mouse liver using a fluorescence-activated cell sorter assay." Anal. Biochem. 334(2): 227-33.
  • Rudensky B., Paz E., Altarescu G., (2003) "Fluorescent flow cytometric assay: a new diagnostic tool for measuring beta-glucocerebrosidase activity in Gaucher disease." Blood Cells Mol. Dis. 30(1): 97-9.
  • Daniels L.B., Glew R.H., Diven W.F., Lee R.E., Radin N.S., (1981) "An improved fluorimetric leukocyte b-glucosidase assay for Gauchers disease." Clin. Chim. Acta 115: 369-375.
  • Kaxpova E.A., Voznyi Ya V., Dudukina T.V., Tsvetkova I.V., (1991) "4-Trifluoromethylumbelliferyl glycosides as new substrates for revealing diseases connected with hereditary deficiency of lysosome glycosidases." Biochem. Int. 24: 1135-1144.
>Show more
>Show less

Click here for Newsletter articles about this product


Technical Support

Question about this product? Ask a Scientist!

We pride ourselves on the high quality of our products and want you to get the best possible results from your assays. If you have any questions about this product or need help optimizing your protocol check out the product FAQs below or ask your own question and one of our expert scientists will get back to you asap:


There are currently no frequently asked questions for this product, click the button above to ask a question