2', 7'-Dichlorofluorescein di-β-D-galactopyranoside (DCFDG)

Product ID: M1194

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Highly sensitive fluorescent substrate for measuring galactosidase and galactocerebrosidase activity inside of live cells and lysosomes.

This substrate releases the highly fluorescent fluorophore 2',7'-dichlorofluorescein (EX: 495nm/EM: 529 nm) at the site of galactosidase or galactocerebrosidase activity. Since the pKa of the released fluorophore is significantly lower than comparable fluorophores, it can retain appreciably more fluorescence in the highly acidic environment of the lysosome than other similar fluorophores. Note: Absorption prior to enzyme hydrolysis is 290 nm; 4.7K).

Technical Data
SKU M1194
Unit Size 25mg
Alternative Names Dichlorofluorescein di-Galactoside, DCFDG
Absorption 495nm
Extinction 529
Detection Method Fluorescence
Molecular Formula C₃₂H₃₀Cl₂O₁₅
Molecular Weight 725.48
Soluble In DMSO, DMF, sl. H2O
Storage Conditions -20C, Desiccated, Protect From Light
Notes High Purity Grade (>99%). Absorption and Emission were measured when product released fluorophore

References and Citations


  • van Es H.H., Veldwijk M., Havenga M., Valerio D. (1997) "A flow cytometric assay for lysosomal glucocerebrosidase" Anal. Biochem. 247: 268-271.
  • Chan KW., Waire J., Simons B., (2004) "Measurement of lysosomal glucocerebrosidase activity in mouse liver using a fluorescence-activated cell sorter assay." Anal. Biochem. 334(2): 227-33.
  • Rudensky B., Paz E., Altarescu G., (2003) "Fluorescent flow cytometric assay: a new diagnostic tool for measuring beta-glucocerebrosidase activity in Gaucher disease." Blood Cells Mol. Dis. 30(1): 97-9.
  • Daniels L.B., Glew R.H., Diven W.F., Lee R.E., Radin N.S., (1981) "An improved fluorimetric leukocyte b-glucosidase assay for Gauchers disease." Clin. Chim. Acta 115: 369-375.
  • Kaxpova E.A., Voznyi Ya V., Dudukina T.V., Tsvetkova I.V., (1991) "4-Trifluoromethylumbelliferyl glycosides as new substrates for revealing diseases connected with hereditary deficiency of lysosome glycosidases." Biochem. Int. 24: 1135-1144.
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