Dihydrorhodamine 123

Product ID: M0545

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Fluorescent peroxidase substrate. One of the most widely used Reactive Oxygen Species (ROS) probes for intracellular measurement and analysis.

DHR123 is one of the most widely used Reactive Oxygen Species (ROS) probes for intracellular measurement and analysis of oxidative species. DHR 123 is an uncharged and nonfluorescent probe that can passively diffuse across membranes where it is oxidized to the cationic rhodamine 123 and localizes in the mitochondria exhibiting a green fluorescence in direct correlation to reactive oxygen species (ROS) present due to cell stress, injury, disease or toxicity. Marker Gene's cell biology grade is a standard around the world for these assays.

Technical Data
SKU M0545
CAS Number 109244-58-8
Unit Size 2mg
Alternative Names DHR123
Absorption 300nm
Detection Method Fluorescence
Molecular Formula C₂₁H₁₈N₂O₃
Molecular Weight 346.39
Soluble In DMSO, CH₂Cl₂, MeOH
Storage Conditions -20C, Desiccated, Protect From Light
Notes Cleavage product: Upon enzymatic (peroxidase) reaction or chemical oxidation, the brightly fluorescent Rhodamine 123 is produced (EX: 505 nm/EM: 534 nm, e=98K/mole).

References and Citations


  • Patterson EK, Fraser DD, Capretta A, Potter RF, Cepinskas G.(2014) "Carbon monoxide-releasing molecule 3 inhibits myeloperoxidase (MPO) and protects against MPO-induced vascular endothelial cell activation/dysfunction." Free Radic Biol Med. 70:167-73.
  • Miyabe C, Miyabe Y, Miura NN, Takahashi K, Terashima Y, Toda E, Honda F, Morio T, Yamagata N, Ohno N, Shudo K, Suzuki J, Isobe M, Matsushima K, Tsuboi R, Miyasaka N, Nanki T. (2013) "Am80, a retinoic acid receptor agonist, ameliorates murine vasculitis through the suppression of neutrophil migration and activation." Arthritis Rheum. 65(2):503-12.
  • Stein VM, Crooks A, Ding W, Prociuk M, O'Donnell P, Bryan C, Sikora T, Dingemanse J, Vanier MT, Walkley SU, Vite CH. (2012) "Miglustat improves purkinje cell survival and alters microglial phenotype in feline Niemann-Pick disease type C." J Neuropathol Exp Neurol. 71(5):434-48.
  • Laird MD, Wakade C, Alleyne Jr CH, Dhandapani KM. (2008)"Hemin-induced necroptosis involves glutathione depletion in mouse astrocytes." Free Radical Biology & Medicine 45: 1103–1114.
  • Genini S, Beltran WA, Stein VM, Aguirre GD.(2014) "Isolation and ex vivo characterization of the immunophenotype and function of microglia/macrophage populations in normal dog retina." Adv Exp Med Biol. 801:339-45
  • Ueda A, Wu CF. (2008) "Effects of hyperkinetic, a beta subunit of Shaker voltage-dependent K+ channels, on the oxidation state of presynaptic nerve terminals." J Neurogenet. 22(2):1-13.
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  • Rothe G, Oser A, Valet G. (1988) "Dihydrorhodamine 123: a new flow cytometric indicator for respiratory burst activity in neutrophil granulocytes." Naturwissenschaften 75: 354-355.
  • Emmendorffer A, Hecht M, Lohmann-Matthes ML, Roesler J. (1990) "A fast and easy method to determine the production of reactive oxygen intermediates by human and murine phagocytes using dihydrorhodamine 123." J Immunol Methods 131: 269-275.
  • Rothe G, Emmendorffer A, Oser A, Roesler J, Valet G. (1991) "Flow cytometric measurement of the respiratory burst activity of phagocytes using dihydrorhodamine 123." J Immunol Methods 138: 133-135.
  • Goossens V, Grooten J, Fiers W. (1996) "The oxidative metabolism of glutamine. A modulator of reactive oxygen intermediate-mediated cytotoxicity of tumor necrosis factor in L929 fibrosarcoma cells." J Biol Chem 271: 192-196.
  • Sobreira C, Davidson M, King MP, Miranda AF. (1996) "Dihydrorhodamine 123 identifies impaired mitochondrial respiratory chain function in cultured cells harboring mitochondrial DNA mutations." J Histochem Cytochem 44: 571-579.
  • Tan S, Sagara Y, Liu Y, Maher P, Schubert D.(1998) "The regulation of reactive oxygen species production during programmed cell death." J Cell Biol 141: 1423-1432.
  • Model MA, KuKuruga MA, Todd RF. (1997) "A sensitive flow cytometric method for measuring the oxidative burst." J Immunol Methods 202: 105-111.
  • Sullivan DM, Wehr NB, Fergusson NM, Levine RL, Finkel T. (2000) "Identification of oxidant-sensitive proteins: TNF-alpha induces protein glutathiolation." Biochem 39: 11121-11128.
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