DiO (3,3-Dioctadecyloxacarbocyanine perchlorate)

Product ID: M1197

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Highly fluorescent membrane probe for labeling live and fixed cells in culture. DiO has been used in long-term neuronal tracing studies.

Long-chain dialkylcarbocyanines are widely used as anterograde and retrograde neuronal tracers in living and fixed tissues and cells. They have been known to stain cells for many cell divisions. They have found use in detection of cell-cell fusion and adhesion, tracing cell migration during development and after transplantation, monitoring lipid diffusion in membranes by FRAP (Fluorescence Recovery After Photobleaching), in cytotoxicity assays and for labeling of lipoproteins.

Technical Data
SKU M1197
CAS Number 34215-57-1
Unit Size 25mg
Alternative Names DiOC18(3); Benzoxazolium, 3-octadecyl-2-[3-(3-octadecyl-2(3H)- benzoxazolylidene)-1-propenyl]-, perchlorate
Absorption 484nm
Emission Wavelength 504
Extinction 139
Detection Method Fluorescence
Molecular Formula C₅₃H₈₅ClN₂O₆
Molecular Weight 881.72
Soluble In CHCl3, DMSO, DMF
Storage Conditions Room Temperature, Desiccated, Protect From Light
Notes High Purity Grade (>99%, TLC)

References and Citations


  • Kim SR, Ho MJ, Lee E, Lee JW, Choi YW, Kang MJ. (2015) "Cationic PLGA/Eudragit RL nanoparticles for increasing retention time in synovial cavity after intra-articular injection in knee joint." Int J Nanomedicine. 10:5263-71.
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  • Gordon S.P., Berezhna S., Scherfeld D., Kahya N., Schwille P. (2005) "Characterization of interaction between cationic lipid-oligonucleotide complexes and cellular membrane lipids using confocal imaging and fluorescence correlation spectroscopy." Biophys. J. 88: 305-16
  • Wu C.C., Reilly J.F., Young W.G., Morrison J.H., Bloom F.E., (2004) "High-throughput morphometric analysis of individual neurons." Cereb. Cortex 14: 543-54
  • Adler M., Tritton T.R., (1988) "Fluorescence depolarization measurements on oriented membranes." Biophys. J. 53: 989-1005.
  • Sondermann J. (1971)"Darstellung Oberflachenaktiver Polymethincyanin-Farbstoffe mit Langen N-Alkyl-Ketten." Liebigs Ann. Chem. 749:183.
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