Product ID: M1843

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DiOC₆(3) is a cell-permeant, lipophilic dye that stains internal membranes of both live and fixed cells, such as the mitochondrial, Golgi and endoplasmic reticulum membranes. Staining patterns are concentration dependent.

DiOC6 (3,3'-dihexyloxacarbocyanine iodide) is a fluorescent dye used for the staining of the endoplasmic reticulum, vesicle membranes and mitochondria. Binding to these structures occurs via the dye's hydrophilic groups. DiOC6 can be used to label living cells, however cell can become quickly damaged due to phototoxicity, so cells stained with this should can only be exposed to light for short periods of time or with low intensity (LED) laser light. When exposed to blue light, DiOC6 fluoresces green. Staining patterns are concentration dependent.

Technical Data
SKU M1843
CAS Number 53213-82-4
Unit Size 100mg
Alternative Names 3,3'-Dihexyloxacarbocyanine Iodide
Emission Wavelength 501
Extinction 484
Detection Method Fluorescence
Molecular Formula C₂₉H₃₇lN₂O₂
Molecular Weight 572.53
Soluble In DMSO
Storage Conditions Room Temperature, Protect From Light, Desiccated

References and Citations


  • Hasmann M, Valet GK, Tapiero H, Trevorrow K, Lampidis T (1989) "Membrane potential differences between adriamycin-sensitive and -resistant cells as measured by flow cytometry." Biochem Pharmacol. 38(2):305-312.
  • Korchak HM, Rich AM, Wilkenfeld C, Rutherford LE, Gerald Weissmann G (1982) "A carbocyanine dye, DiOC₆(3), acts as a mitochondrial probe in human neutrophils."Biochemical and Biophysical Research Communications 108(4): 1495–1501.
  • Chen JC, Zhang X, Singleton TP, Kiechle FL (2004)"Mitochondrial Membrane Potential Change Induced by Hoechst 33342 in Myelogenous Leukemia Cell Line HL-60." Annals of Clinical & Laboratory Science 34(4):458-466.
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