MarkerGene™ dsDNA Assay Kit-for use with the Qubit® fluorometer, 100 samples

Product ID: M1952



Unit SizePriceQuantity 
1kit
$73.16
  • Buy 5 for $58.53 each and save 20%

Availability: In stock


Description

The assay kit measures dsDNA in solution, and is adapted for use with the Qubit® fluorometer.

The accurate quantitation of double-stranded DNA (dsDNA) is critical for a wide variety of molecular biology applications. The traditional method of absorbance measurement at 260 nm (A260 nm) has several well-known disadvantages, including interference from other nucleic acids such as RNA, single-stranded DNA, and nucleotides. The A260 nm method can also be affected by contaminants of nucleic acid preparations such as proteins or phenol. The limited sensitivity and linear range of the A260 nm method (limit of detection ~100-500 ng/mL dsDNA) are further impediments to its performance when working with small amounts of DNA sample.

The MarkerGene™ dsDNA Assay Kits, M1952 (100 samples) and M2000 (500 samples), make DNA quantitation both easy and accurate. A variety of different contaminants, such as RNA, salts, free nucleotides, solvents, detergents, or proteins are well tolerated in the assay.

M1952 provides reagents for measuring up to 100 assays of 200 uL each. M2000 provides reagents for measuring up to 500 assays of 200 uL each. The figure to the left illustrates how the MarkerGene™ dsDNA assay compares to the Life Technologies Qubit® assay when used with the Qubit® fluorometer.


Technical Data
SKU M1952
Unit Size 1kit
Detection Method Fluorescence
Storage Conditions -20C

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References and Citations

References:

  • Batchelor R, Hagen D, Johnson I, Beechem J. (2003) "A fluorescent high-throughput assay for double-stranded DNA: the RediPlate PicoGreen assay." Comb Chem High Throughput Screen 6: 287-291.
  • Bolger R, Lenoch F, Allen E, Meiklejohn B, Burke T. (1997) "Fluorescent dye assay for detection of DNA in recombinant protein products." Biotechniques 23: 532-537.
  • Rye HS, Dabora JM, Quesada MA, Mathies RA, Glazer AN. (1993) "Fluorometric assay using dimeric dyes for double- and single-stranded DNA and RNA with picogram sensitivity." Anal Biochem 208:144-50.
  • Glazer AN, Rye HS. (1992) "Stable dye-DNA intercalation complexes as reagents for high-sensitivity fluorescence detection." Nature 359: 859-861.
  • Burres NS, Frigo A, Rasmussen RR, McAlpine JB. (1992) "A colorimetric microassay for the detection of agents that interact with DNA." J Nat Prod 55: 1582-1587.
  • Moreno LA, and Cox KL. (2010) "Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen dye." J Vis Exp 45: 2465.
  • Vitzthum F, Geiger G, Bisswanger H, Brunner H, Bernhagen J. (1999) "A quantitative fluorescence-based microplate assay for the determination of double-stranded DNA using SYBR Green I and a standard ultraviolet transilluminator gel imaging system." Anal Biochem 276: 59-64.
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