MarkerGene™ Dyrect JC-1 Mito Health Assay Kit

Product ID: M1890



Unit SizePriceQuantity 
1kit
$262.60
  • Buy 5 for $210.08 each and save 21%

Availability: In stock


Description

Utilizes the live cell carbocyanine dye JC-1 which indicates changes in ΔΨm, the electrochemical gradient across the mitochondrial membranes by reversibly changing its emission from green in non-polar mitochondria to orange/red J aggregates in hyperpolarized mitochondria.

This kit provides 5 ready to use vials of JC-1 to stain mitochondria in live cells. Samples can be stained in complete media to minimize disruption of experimental conditions or in the supplied 5X concentrate Opti-Klear™ Live Cell Imaging Buffer (M1898)to reduce the fluorescence background associated with most media. Useful in nearly all cell types tested, JC-1 remains one of the most reliable markers of mitochondrial health in live cells.

Technical Data
SKU M1890
Unit Size 1kit
Detection Method Fluorescence
Storage Conditions -20C, Protect From Light

References and Citations

References:

  • Smiley ST, Reers M, Mottola-Hartshorn C, Lin M, Chen A, Smith TW, Steele GD Jr, Chen LB. (1991) “Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1.” Proc Natl Acad Sci U S A 88(9):3671-5.
  • Reers M, Smith TW, Chen LB. (1991) “J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential.” Biochemistry. 30(18):4480-6.
  • Reers M, Smiley ST, Mottola-Hartshorn C, Chen A, Lin M, Chen LB. (1995) “Mitochondrial membrane potential monitored by JC-1 dye.” Methods Enzymol. 1995;260:406-17.
  • Wallace DC. (1999) “Mitochondrial diseases in man and mouse.” Science, 283 (5407): 1482-88.
  • Mathur A, Hong Y, Kemp BK, Barrientos AA, Erusalimsky JD. (2000) Evaluation of fluorescent dyes for the detection of mitochondrial membrane potential changes in cultured cardiomyocytes.” Cardiovascular Research 46 (1): 126-138.
  • Salvioli S, Ardizzoni A, Franceschi C, Cossarizza A. (1997) “JC-1, but not DiOC(6)(3) or rhodamine 123, is a reliable fluorescent probe to assess Delta Psi changes in intact cells: Implications for studies on mitochondrial functionality during apoptosis.” FEBS Letters, vol. 411 (1): 77-82.
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