Eosin 5-isothiocyanate

Product ID: M1074



Unit SizePriceQuantity 
25mg
$59.85
  • Buy 5 for $47.88 each and save 20%

Availability: In stock


Description

This long wavelength red fluorescent labeling derivative can be used to label amino groups on proteins. It can also be used as a reversible inhibitor of the erythrocyte calcium pump as well as an effective phosphorescent probe in protein rotational studies.

This long wavelength red fluorescent labeling derivative can be used to label amino groups on proteins. It can also be used as a reversible inhibitor of the erythrocyte calcium pump as well as an effective phosphorescent probe in protein rotational studies. Eosin derivatives are also used in diaminobenzidine photo-oxidation processes and fluorescence resonance energy transfer (FRET) systems.

Technical Data
SKU M1074
CAS Number 60520-47-0
Unit Size 25mg
Absorption 520nm
Emission Wavelength 544
Extinction 95
Detection Method Fluorescence
Molecular Formula C₂₁H₇Br₄NO₅S
Molecular Weight 704.97
Soluble In DMF (pH > 6 )
Storage Conditions 2-8C, Desiccated, Protect From Light
Notes Eosin derivatives also exhibit phosphorescence with an emission maximum at ~680 nm.

References and Citations

Citations:

  • Asselin J, Legros P, Grégoire A, Boudreau D (2016) "Correlating Metal-Enhanced Fluorescence and Structural Properties in Ag@SiO2 Core-Shell Nanoparticles" Plasmonics:1-8.
  • Kaastrup K, Sikes HD (2015) "Investigation of dendrimers functionalized with eosin as macrophotoinitiators for polymerization-based signal amplification reactions" RSC Adv. 5, 15652-15659
  • Brouard D, Ratelle O, Guillermo Bracamonte A, St-Louis M, Boudreau D. (2013) "Direct molecular detection of SRY gene from unamplified genomic DNA by metal-enhanced fluorescence and FRET." Anal. Method 5: 6896-6899.
  • Rainville L, Dorais MC, Boudreau D (2013)" Controlled synthesis of low polydispersity Ag@SiO2 core–shell nanoparticles for use in plasmonic applications" RSC Adv. 3: 13953–13960
  • Shigeoka Y, Fujisawa T, Teshiba S, Fukumori H, Yamamoto K, Banno Y, Aso Y. (2013)"Salt-induced changes in the subunit structure of the Bacillus stearothermophilus lipoate acetyltransferase." Biosci Biotechnol Biochem. 77(8):1637-44.
  • Brouard D, Viger ML, Bracamonte AG, Boudreau D (2011) "Label-Free Biosensing Based on Multilayer Fluorescent Nanocomposites and a Cationic Polymeric Transducer" ACS Nano, 5 (3), 1888–1896
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References:

  • Hulspas R, Krijtenburg PJ, Keij JF, Bauman JG. (1993) "Avidin-EITC: an alternative to avidin-FITC in confocal scanning laser microscopy." J. Histochem. Cytochem. 41: 1267-1272.
  • Wagner R., et al. (1988) "The Binding of Eosin-Labeled Subunit S to the Isolated Chloroplast ATPase, CF1, as Revealed by Rotational Diffusion in Solution." FEBS Lett. 230: 109.
  • Andreo C.S., et al. (1986) "Chemical Modification of the Phosphoenolpyruvate Carboxylase from Maize Leaves and Its Conformation in Isotropic Solution. Studies Via Triplet Lifetime and Rotational Diffusion Using Eosin Isothiocyanate as Label." Biochim Biophys Acta 870: 292.
  • Chiba T, Sato Y, Suzuki Y. (1986) "Amino acid residues complexed with eosin 5-isothiocyanate in band 3 protein of the human erythrocyte." Biochim. Biophys. Acta 858: 107-117.
  • Sato Y, Chiba T, Suzuki Y. (1985) "Characteristics of anion transport channels in the human erythrocyte. I. Interactions between eosin 5-isothiocyanate and band 3 proteins." Chem. Pharm. Bull. (Tokyo) 33: 3935-3944.
  • Eads TM, Thomas DD, Austin RH. (1984) "Microsecond rotational motions of eosin-labeled myosin measured by time-resolved anisotropy of absorption and phosphorescence." J. Mol. Biol. 179: 55-81.
  • Wilderspin AF, Green NM. (1983) "The reaction of fluorescein isothiocyanate with thiols: a method for assay of isothiocyanates." Anal. Biochem. 132: 449-455.
  • Wagner R, Junge W. (1982) "Coupling factor for photophosphorylation labeled with eosin isothiocyanate: activity, size, and shape in solution." Biochemistry 21: 1890-1899.
  • Johnson P, Garland PB. (1982) "Fluorescent triplet probes for measuring the rotational diffusion of membrane proteins." Biochem J 203: 313-321.
  • Nigg E, Kessler M, Cherry RJ. (1979) "Labeling of human erythrocyte membranes with eosin probes used for protein diffusion measurements: inhibition of anion transport and photo-oxidative inactivation of acetylcholinesterase." Biochim. Biophys. Acta 550: 328-340.
  • Contillo L.G., et al. (1994) "Screening of Protease Inhibitors." Techniques in Protein Chemistry V, Crabb J.W., Ed. pp. 493-500.
  • Cherry RJ, Cogoli A, Oppliger M, Schneider G, Semenza G. (1976) "A spectroscopic technique for measuring slow rotational diffusion of macromolecules. 1: Preparation and properties of a triplet probe."Biochemistry. 15(17):3653-3656.
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