Eosin-5-thioureidyl-m-phenylboronic acid, triethylammonium salt

Product ID: M1202

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Long-wavelength fluorescent labeling agent for cis-diols (sugars) at high pH values on cell surfaces and in vitro for carbohydrate determination in glycoproteins and for HPLC analysis of sugars.

This labeling agent can be used in conjunction with other fluorophores, for multiplexed analysis, in fluorescence energy transfer (FRET) studies or in a high-throughput format (EX 520/EM 544). The fluorescence emission of the eosin labeled compound can exhibit environment sensitive fluorescence emission properties. The eosin derivatives can also exhibit phosphorescence with an emission maximum at ~680 nm. It has found extensive use in detection of glycosylation of hemoglobin for diabetes mellitis diagnosis. See the references below for more information.

Technical Data
SKU M1202
Unit Size 25mg
Alternative Names Eosin m-aminophenylboronic acid
Absorption 520nm
Emission Wavelength 544
Detection Method Fluorescence
Molecular Formula C₃₉H₄₅BBr₄O₇N₄S
Molecular Weight 1044.29
Soluble In DMSO, DMF, sl. H2O
Storage Conditions -20C, Desiccated, Protect From Light

References and Citations


  • Luis GP. (1998) "Selective fluorescent chemosensor for fructose." Analyst 123: 155.
  • Blincko S, Edwards R. (1998) "Non-separation assay for glycohemoglobin." Clinical Chemistry 44:6 1302-1308.
  • Gamoh K. (1990) "Determination of traces of natural brassinosteroids as dansylaminophenylboronates by liquid chromatography with fluorometric detection." Anal. Chim. Acta 228: 101.
  • Hayashi Y, Makino M. (1985) "Fluorometric measurement of glycosylated albumin in human serum." Clin. Chim. Acta 149: 13-19.
  • Vainio P. (1983) "N-(5-Dimethylaminonaphthalene-1-sulfonyl)-3-aminobenzene boronic acid as an active-site-directed fluorescent probe of lipoprotein lipase." Biochim. Biophys. Acta 746: 217-219.
  • Burnett TJ, Peebles HC, Hageman JH. (1980) "Synthesis of a fluorescent boronic acid which reversibly binds to cell walls and a diboronic acid which agglutinates erythrocytes." Biochem. Biophys. Res. Commun. 96: 157-162.
  • Turner APF, Chena B, Piletsky SA. (1999) "In vitro diagnostics in diabetes: meeting the challenge." Clinical Chemistry. 45: 1596-1601.
  • Boeseken J. (1949) "The use of boric acid for the determination of configuration of carbohydrates." Adv. Carbo. Chem. 4: 189-210.
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