Cell permeant esterase substrates are often used for cell viability and cytotoxicity assays in a wide variety of animal cells, as well as bacteria and yeast. These fluorogenic substrates measure enzyme activity and cell-membrane integrity, the latter of which is required for intracellular retention of their fluorescent products upon enzymatic cleavage. Applications of esterase substrates parallel those of 51Cr-release assays without the risks associated with the use of radioactive materials, making them a safer and more effective alternative to traditional methods.

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  1. M0011

    5(6)-Carboxyfluorescein diacetate (CFDA)


    Popular, ultrasensitive, fluorescent substrate for measuring esterase activity in live cells often used in cell viability assays and "Live-Dead" assay systems, especially in flow cytometry.

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  2. 5(6)-Carboxyfluorescein diacetate, NHS ester

    5(6)-Carboxyfluorescein diacetate, NHS ester


    Cell cycle, cell viability probe useful for cell labeling, especially in flow cytometry. Learn More
  3. Fluorescein diacetate (FDA)

    Fluorescein diacetate (FDA)


    FDA is one of the most widely-used green fluorogenic substrates for esterase activity. FDA is also a common viability stain for plant cells, protoplasts and tissues. Learn More
  4. Resorufin acetate

    Resorufin acetate


    Common long-wavelength esterase substrate, useful in cell viability assays, or for high throughput screening of esterase activity in a live cell format. Learn More
  5. 3-(2-Benzoxazolyl)umbelliferyl acetate

    3-(2-Benzoxazolyl)umbelliferyl acetate


    Fluorogenic esterase substrate with blue-green emission (468 nm) upon enzyme activity. Learn More
  6. Casein-FITC conjugate

    Casein-FITC conjugate


    A conjugated protein substrate used for fluorometric measurement of protease activity in vitro or for use in endocytosis labeling and analysis. Learn More
  7. Green CMFDA

    Green CMFDA


    Cell permeable green fluorescent dye. Learn More
  8. 5-Carboxyfluorescein diacetate succinimidyl ester

    5-Carboxyfluorescein diacetate succinimidyl ester


    Amine-reactive non-fluorescent probe for conjugation to any protein or peptide. Learn More

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