Esterase

Cell permeant esterase substrates are often used for cell viability and cytotoxicity assays in a wide variety of animal cells, as well as bacteria and yeast. These fluorogenic substrates measure enzyme activity and cell-membrane integrity, the latter of which is required for intracellular retention of their fluorescent products upon enzymatic cleavage. Applications of esterase substrates parallel those of 51Cr-release assays without the risks associated with the use of radioactive materials, making them a safer and more effective alternative to traditional methods.

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  1. M0011

    5(6)-Carboxyfluorescein diacetate (CFDA)

    $140.24

    Popular, ultrasensitive, fluorescent substrate for measuring esterase activity in live cells often used in cell viability assays and "Live-Dead" assay systems, especially in flow cytometry.

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  2. 5(6)-Carboxyfluorescein diacetate, NHS ester

    5(6)-Carboxyfluorescein diacetate, NHS ester

    $149.61

    Cell cycle, cell viability probe useful for cell labeling, especially in flow cytometry. Learn More
  3. Fluorescein diacetate (FDA)

    Fluorescein diacetate (FDA)

    $59.85

    FDA is one of the most widely-used green fluorogenic substrates for esterase activity. FDA is also a common viability stain for plant cells, protoplasts and tissues. Learn More
  4. Resorufin acetate

    Resorufin acetate

    $81.59

    Common long-wavelength esterase substrate, useful in cell viability assays, or for high throughput screening of esterase activity in a live cell format. Learn More
  5. 3-(2-Benzoxazolyl)umbelliferyl acetate

    3-(2-Benzoxazolyl)umbelliferyl acetate

    $108.20

    Fluorogenic esterase substrate with blue-green emission (468 nm) upon enzyme activity. Learn More
  6. Casein-FITC conjugate

    Casein-FITC conjugate

    $88.55

    A conjugated protein substrate used for fluorometric measurement of protease activity in vitro or for use in endocytosis labeling and analysis. Learn More
  7. Green CMFDA

    Green CMFDA

    $176.22

    Cell permeable green fluorescent dye. Learn More
  8. 5-Carboxyfluorescein diacetate succinimidyl ester

    5-Carboxyfluorescein diacetate succinimidyl ester

    $198.00

    Amine-reactive non-fluorescent probe for conjugation to any protein or peptide. Learn More

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