Enzyme Substrates and Kits for Esterase

Cell permeant esterase substrates are often used for cell viability and cytotoxicity assays in a wide variety of animal cells, as well as bacteria and yeast. These fluorogenic substrates measure enzyme activity and cell-membrane integrity, the latter of which is required for intracellular retention of their fluorescent products upon enzymatic cleavage.¹ Applications of esterase substrates parallel those of ⁵¹Cr-release assays without the risks associated with the use of radioactive materials, making them a safer and more effective alternative to traditional methods.^2,3

FLUOROGENIC SUBSTRATES

PRODUCT #	PRODUCT NAME	COLOR OF RELEASED PRODUCT (Abs/Em) (nm)
M0011	5(6)-Carboxyfluorescein diacetate (CFDA) (also see our Live:Dead Assay Kit, M0795, for more details)	Green (492/517)
M0013	5(6)-Carboxyfluorescein diacetate-NHS Ester	Green (492/517)
M0060	Fluorescein diacetate (FDA)	Green (490/514)
M0780	Rose Bengal Diacetate^4	Red (561/572 (MeOH))

REFERENCES:

1. Hayes, A.W., Ed., pp. 1231-1258 (1994)

2. Hum Immunol 37, 264 (1993)

3. J Histochem Cytochem 33, 77 (1985)

4. Photochem Photobiol 66, 374 (1997)