Enzyme Substrates and Kits for Esterase

Cell permeant esterase substrates are often used for cell viability and cytotoxicity assays in a wide variety of animal cells, as well as bacteria and yeast. These fluorogenic substrates measure enzyme activity and cell-membrane integrity, the latter of which is required for intracellular retention of their fluorescent products upon enzymatic cleavage. Applications of esterase substrates parallel those of 51Cr-release assays without the risks associated with the use of radioactive materials, making them a safer and more effective alternative to traditional methods.
Product ID Product Name Description
M0011 5(6)-Carboxyfluorescein diacetate (CFDA)  CFDA is a popular, ultrasensitive, fluorescent substrate for measuring esterase activity in live cells. It is often used in cell viability assays and "Live-Dead" assay systems, especially automated cell viability assays, because the cleavage product produced upon enzymatic or chemical hydrolysis of the acetate group(s) is carboxyfluorescein Product M0122. This fluorophore is well-retained inside live cells, while the substrate CFDA is membrane permeant. See the references for additional information about CFDA use in bacterial, plant, yeast and mammalian cell culture. 
M0013 5(6)-Carboxyfluorescein diacetate, NHS ester  Labeling reagent (amine-reactive) with green emission spectral properties (Abs 488 nm / EM 517 nm) after removal of acetate esters, intracellularly.  
M0060 Fluorescein diacetate (FDA)  USP Grade. The most widely-used green fluorogenic substrate for esterase activity. 
M0780 Rose Bengal Diacetate  Rose bengal is a per-halogenated fluorescein derivative that is among the most efficient producers of singlet oxygen known. The diacetate derivative M0780, exhibits greatly improved membrane permeability, allowing it to accumulate intracellularly where esterase activity releases the active rose bengal photosensitizer. Activation with UV light at 561 nm causes singlet oxygen production and photosensitization. Rose Bengal has been widely used in photodynamic therapy of tumors, to inactivate viruses, gram-positive bacteria and protozoa, and to produce photohemolysis, and induce occlusion of blood vessels in a procedure called photothrombosis. 
M0806 Resorufin Acetate  Resorufin acetate is a common long-wavelength esterase substrate, useful in cell viability assays, or for high throughput screening of esterase activity in a live cell format. Resorufin acetate is also a substrate for sheep liver cytosolic aldehyde dehydrogenase (CAD) as well as for assay of chymotrypsin activity, where the absorbance of the product resorufin Product M0202 is several times more intense than that formed by the widely used p-nitrophenyl acetate. 
M0807 2', 7'-Dichlorofluorescin Diacetate  This reduced derivative of 2',7'-dichlorofluorescein is a cell permeable reagent for use in detection of peroxidase activity (oxidative metabolism, intracellular regulation of reactive oxygen species or oxidative burst) inside living cells. Upon enzyme activity, the highly fluorescent dye 2',7'-dichlorofluorescein Product M1278 is produced, with EX: 495nm and EM: 529 nm. See also Product M0545. 
M1253 3-(2-Benzoxazolyl)umbelliferyl acetate  Fluorogenic esterase substrate with blue-green emission (468 nm) upon enzyme activity.  
     
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