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Enzyme Substrates and Kits Enzyme Substrates and Kits
Enzyme Inhibitors Enzyme Inhibitors
MGT Detection Kits MGT Detection Kits
Cell Regulatory Substrates Cell Regulatory Substrates
Special Labeling Reagents Special Labeling Reagents
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Carbohydrates/Oligosaccharides Carbohydrates/Oligosaccharides  
Fluorescent Reagents Fluorescent Reagents
Enzyme Substrates and Kits for Esterase

Cell permeant esterase substrates are often used for cell viability and cytotoxicity assays in a wide variety of animal cells, as well as bacteria and yeast. These fluorogenic substrates measure enzyme activity and cell-membrane integrity, the latter of which is required for intracellular retention of their fluorescent products upon enzymatic cleavage. Applications of esterase substrates parallel those of 51Cr-release assays without the risks associated with the use of radioactive materials, making them a safer and more effective alternative to traditional methods.

  ID   Name   CAS Unit Size Price Bulk Price (5+)
M0780   Rose bengal diacetate 61738-01-0 5 mg 86.80 69.44
Rose bengal is a per-halogenated fluorescein derivative that is among the most efficient producers of singlet oxygen known. This diacetate derivative exhibits greatly improved membrane permeability, allowing it to accumulate intracellularly where esterase activity releases the active rose bengal photosensitizer.
M0806   Resorufin acetate 1152-14-3 25 mg 63.13 50.50
Common long-wavelength esterase substrate, useful in cell viability assays, or for high throughput screening of esterase activity in a live cell format.
M0060   Fluorescein diacetate (FDA) 596-09-8 1 g 46.30 37.04
Most widely-used green fluorogenic substrate for esterase activity.
M0807   2', 7'-Dichlorofluorescin diacetate 4091-99-0 25 mg 46.30 37.04
Reduced derivative of 2',7'-dichlorofluorescein is a cell permeable reagent for use in detection of peroxidase activity (oxidative metabolism, intracellular regulation of reactive oxygen species or oxidative burst) inside living cells.
M0013   5(6)-Carboxyfluorescein diacetate, NHS ester 150347-59-4 25 mg 115.75 92.60
Labeling reagent (amine-reactive) with green emission spectral properties (Abs 488 nm / EM 517 nm) after intracellular removal of acetate esters.
M0011   5(6)-Carboxyfluorescein diacetate (CFDA) 124387-19-5 100 mg 108.50 86.80
Popular, ultrasensitive, fluorescent substrate for measuring esterase activity in live cells. It is often used in cell viability assays and "Live-Dead" assay systems
M1253   3-(2-Benzoxazolyl)umbelliferyl acetate 97004-78-9 25 mg 83.71 66.97
Fluorogenic esterase substrate with blue-green emission (468 nm) upon enzyme activity.

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