Ethidium homodimer (EthD-1)

Product ID: M1093

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High affinity fluorescent nucleic stain. It binds to both DNA and RNA in a sequence-independent manner and with a >30-fold fluorescence enhancement.

Ethidium homodimer is a high affinity fluorescent nucleic stain. It binds to both DNA and RNA in a sequence-independent manner with a >30-fold fluorescence enhancement upon binding. The DNA binding of each ethidium homodimer molecule covers four base pairs and is believed to occur by intercalation. Because the dye is highly positively charged, it cannot cross cell membranes to stain living cells and is therefore a common "dead" cell marker used in numerous cell biology, microscopy and flow cytometry applications.

Technical Data
SKU M1093
CAS Number 61926-22-5
Unit Size 1mg
Emission Wavelength 617
Detection Method Fluorescence
Molecular Formula C₄₆H₅₀Cl₄N₈
Molecular Weight 856.78
Soluble In DMSO, DMF
Storage Conditions -20C, Protect From Light
Notes Ex: 487/528 nm (wo/w DNA)

References and Citations


  • Harapanhalli RS, Matalka KZ, Jones PL, Mahmood A, Adelstein SJ, Kassis A. (1998) "Lysine-directed conjugation of ethidium homodimer to B72.3 antibody: retention of immunoreactivity but altered tumor targeting." Nuclear Medicine and Biology 25(3): 267-78.
  • Zakharov SF, Garner MM, Chrambach A. (1995) "Fluorescent labeling of DNA with ethidium homodimer without measurable decrease in DNA mobility: application to automated gel electrophoresis apparatus." Anal Biochem 2241: 195-8.
  • Wagner E, Cotten M, Mechtler K, Kirlappos H, Birnstiel ML. (1991) "DNA-binding transferrin conjugates as functional gene-delivery agents: synthesis by linkage of polylysine or ethidium homodimer to the transferrin carbohydrate moiety." Bioconjug Chem. Jul-Aug;2(4):226-31.
  • Rye HS, Quesada MA, Peck K, Mathies RA, Glazer AN. (1991) "High-sensitivity two-color detection of double-stranded DNA with a confocal fluorescence gel scanner using ethidium homodimer and thiazole orange" Nucleic Acids Research 19(2): 327-33.
  • Glazer AN, Peck K, Mathies RA. (1990) "A stable double-stranded DNA-ethidium homodimer complex: application to picogram fluorescence detection of DNA in agarose gels" Proceedings of the National Academy of Sciences of the United States of America 87(10): 3851-5.
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