MarkerGene™ FACS Fluorescent Blue lacZ β-Galactosidase Detection Kit
Product ID: M0255
This flow cytometry kit can be used in conjunction with eGFP or RFP assays since it allows highly sensitive detection of β-galactosidase activity in mammalian, yeast, bacterial, or plant cells, at a shorter wavelength (blue fluorescence) than other methods. It is especially amenable to co-staining with a green (fluorescein, GFP) labeling for dual labeling experiments.
Although chromogenic assays of beta-galactosidase activity (i.e. X-Gal) have use, application of the fluorogenic substrate 3-carboxyumbelliferyl β-D-galactopyranoside (CUG) combined with Fluorescence Activated Cell Sorting (FACS) analysis has been shown to be several orders of magnitude more sensitive. In addition, because of its high water solubility and detection limits, the CUG substrate has found extensive use in automated ELISA type assay systems. This assay is especially useful when co-staining with a fluorescein-type cell label (FITC-antibody, fluorescein based substrate, etc.) for dual wavelength detection. Many new instruments have multi-color FACS detection capabilities (see the BD LSRII system for example), that can utilize this new kit. Please consult your instrument manufacturer brochure for more information.
The FACS Blue lacZ β-Galactosidase Detection kit provides all the reagents and a detailed protocol to perform up to 500 automated (5 x 96 well microtiterplate) assays.
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