Fluorescein di-β-D-glucopyranoside (FDGlu)

Product ID: M0881

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Ultrasensitive fluorogenic substrate for quantitative measurement of β-glucosidase activity in vivo or in vitro.

This fluorogenic β-glucosidase substrate releases the highly fluorescent dye fluorescein (EX 488 nm/EM 512 nm) in direct correlation with enzyme activity. It has been used for analysis of cell viability and in diabetes research.

Technical Data
SKU M0881
CAS Number 129787-66-2
Unit Size 5mg
Absorption 300nm
Emission Wavelength 514
Detection Method Fluorescence
Molecular Formula C₃₂H₃₂O₁₅
Molecular Weight 656.6
Soluble In DMSO, MeOH, H2O
Storage Conditions -20C, Desiccated
Notes Emission of the fluorescein fluorophore (EX 490nm/EM 514nm) released upon beta-glucosidase enzyme activity.

References and Citations


  • Nakamura K, Iizuka R, Nishi S, Yoshida T, Hatada Y (2016) "Culture-independent method for identification of microbial enzyme-encoding genes by activity-based single-cell sequencing using a water-in-oil microdroplet …" Scientific Reports 6.
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  • "An in situ study of beta-glucosidase activity in normal and Gaucher fibroblasts with fluorogenic probes." Kohen, E., Kohen, C., Hirschberg, J.G., Santus, R., Grabowski, G., Mangel, W., Gatt, S., Prince, J. Cell Biochem Funct 11, 167 (1993)
  • "Yeast exo-beta-glucanases can be used as efficient and readily detectable reporter genes in Saccharomyces cerevisiae." Cid, V.J., Alvarez, A.M., Santos, A.I., Nombela, C., Sanchez, M. Yeast 10, 747 (1994)
  • "A flow cytometric assay for lysosomal glucocerebrosidase." Van Es, H.H., Veldwijk, M., Havenga, M., Valerio, D. Anal Biochem 247, 268 (1997)
  • "Nucleic acid detection using non-radioactive labelling methods." Mansfield, E.S., Worley, J.M., McKenzie, S.E., Surrey, S., Rappaport, E., Fortina, P. Mol Cell Probes 9, 145 (1995)
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