Filter Sets

Analyte Dye Excitation Max. (nm) Emission Max. (nm) Omega Filter Sets Chroma Filter Sets Nikon Filter Sets* Zeiss Filter Sets

DiR (DiIC18(7))

750

779

XF112

41009

 

 

DiD (DiIC18(5))

644

665

XF110
XF47

41008
31023

 

 

Allophycocyanin

650

660

XF44
XF110

41013
31006

 

Filter Set # 26

7-Aminoactinomycin D (7-AAD)

546

647

XF103
XF35

41005

All Y

Filter Set # 00

Fura Red

472 (low [Ca2+])
436 (high [Ca2+])

657 (low [Ca2+])
637 (high [Ca2+])

XF17

71003

BV-1A

BV-2A

BV-2B

B-1A

B-2A

B-3A

 

Di-8-ANEPPS, Di-4-ANEPPS 

468

635

XF17

71006

B-1A

B-2A

B-3A

Filter Sets # 09, 16 (Di-8-ANNEPS)

RH 414

500

635

XF21

31011

 

Filter Set # 14

JC-9

506

525 (monomer)
635 (aggregate)

XF26

71010

 

 

Nile red

549

628

XF103
XF35

41005
31014

Any G

Any Y

Filter Set # 31

Ethidium homodimer (DNA complex)

528

617

XF103
XF35

41005
31005

Any G

Filter Set # 00

Propidium iodide

536

617

XF103
XF35

41005
31005

Any G

Any Y

Filter Set # 00

Texas Red and Texas Red-X-conjugates

595

615

XF102
XF43

41004
31004

G-1A

G-2B

Any Y

Filter Set # 00

Ethidium bromide (DNA complex)

518

605

XF103
XF33

41006
31008

Any G

Filter Sets # 14, 43

ROX

580

605

XF102
XF41

41034
31004

Any Y

Filter Set # 45

X-rhod-1 indicator

580

602

XF102
XF41

41034
31004

Any Y

 

JC-1

498 (monomer)
593 (aggregate)

525 (monomer)
595 (aggregate)

XF26

71010

Any B

G-2A

G-2B

G-2E/C

Cy3

 

DiA 

456

590

XF21

31024

BV-2A

BV-2B

B-1A

B-2A

B-3A

Filter Sets # 09, 16

Lissamine rhodamine B

570

590

XF102
XF41

41034
31004

Y-2E/C

Filter Set # 45

Rhodamine Red

570

590

XF102
XF41

41034
31004

Any Y

Filter Set # 31

Resorufin

571

585

XF39

41010

Y-2E/C

 

Tetramethylrhodamine, Rhodamine B

555

580

XF108
XF32

41002
31002

G-1A

G-2A

G-1B

G-2E/C

Cy3

Y-2E/C

Filter Set # 20

Rhod-2

552

578

XF108
XF32

41002
31002

Y-2E/C

Filter Set # 20

R-phycoerythrin

565

575

XF108
XF32

41002
31014

 

Filter Sets # 14, 43

DiI (DiIC18(3))

549

565

XF108
XF32

41002
31002

G-1A

G-2A

G-1B

G-2E/C

Cy3

Filter Set # 20

6-Carboxyrhodamine 6G

525

555

XF104
XF99

41011

 

 

JOE dye

525

555

XF104
XF99

41011

G-1A

G-2A

G-1B

G-2E/C

Cy3

 

Cascade Yellow

402

545

XF14

31038

UV-1A

UV-2A

V-1A

V-2A

Filter Sets # 05, 06

Eosin

524

544

XF104

41011

G-1A

G-2A

G-1B

G-2E/C

Cy3

 

Lucifer yellow CH

428

536

XF96
XF14

32000
31010

V-2A

BV-1A

BV-2A

BV-2B

B-2A

B-3A

B-2E

Filter Sets # 05, 06, 37, 48

NBD (amine conjugates)

465

535

XF100
XF23

41001
31018

BV-1A

BV-2A

BV-2B

Filter Set # 37

Magnesium Green

506

531

XF104
XF23

41028
31001

Any B

Filter Set # 46

Rhodamine 123

507

529

XF104
XF23

41028
31001

Any B

Filter Set # 46

BCECF

482 (low pH)
503 (high pH)

520 (low pH)
528 (high pH)

XF16

71001

Any B

Filter Sets # 09, 10, 16, 17, 44

Rhodamine Green

502

527

XF100
XF23

41001
31001

Any B

Filter Set # 46

Acridine orange (DNA/RNA complex)

500 (+DNA)
460 (+RNA)

526 (+DNA)
650 (+RNA)

XF104 (DNA)
XF21 (RNA)

41001 (DNA)
31001 (RNA)

Any B (DNA)

B-1A

B-2A

B-3A (RNA)

DNA Only;

     Filter Sets # 16, 17, 46

RNA Only;

     Filter Set # 09

Both DNA and RNA;

     Filter Sets # 09, 10, 16, 17, 37, 38, 44

Fluo-3

506

526

XF104
XF23

41028
31001

Any B

 

Filter Sets # 09,10, 38

Rhodamine 110

496

520

XF100
XF23

41001
31001

Any B

Filter Sets # 09, 10, 16, 17, 37, 44

Fluorescein and FITC conjugates

494

518

XF100
XF23

41001
31001

Any B

Filter Sets # 09, 10, 16, 17, 44

 

Calcein

494

517

XF100
XF23

41001
31001

Any B

Filter Sets # 09, 10, 16, 17, 44

Fluo-4

494

516

XF100
XF23

41001
31001

Any B

Filter Set # 09, 10, 16, 17, 38, 44

Fura-2

363 (low [Ca2+])
335 (high [Ca2+])

512 (low [Ca2+])
505 (high [Ca2+])

XF04

71000

UV-2A

UV-2B

 

DiO (DiOC18(3))

484

501

XF100
XF23

41001
31001

Any B

Filter Sets # 13, 38, 44

Monobromobimane

394

490

XF76

32000

V-1A

V-2A

UV-2A

UV-2B

Filter Sets # 05, 06, 18, 37

2,6-TNS

380

483

XF76

31000

31013

11000v2

V-2A

BFP

Filter Sets #01, 02

DAPI

358

461

XF136
XF06

31000
31013

Any UV

Any V

Filter Sets # 01, 02

Hoechst 33258, Hoechst 33342 (DNA complexes)

352

461

XF136
XF06

31000
31013

Any UV

Any V

Filter Set # 02

Bimane

380

458

XF136
XF06

31000
31013

V-2A

Filter Set # 18

7-Hydroxy-4-methylcoumarin (ummbelliferone)

360

455

XF136
XF06

31000
31013

 

Filter Sets # 01, 02

 

Cascade Blue

400

420

XF113
XF10

31021
31009

UV-1A

UV-2A

V-1A

BFP

Filter Sets # 01, 02

Indo-1

346 (low [Ca2+])
330 (high [Ca2+])

475 (low [Ca2+])
401 (high [Ca2+])

XF07

71002

UV-1A

UV-2A

UV-2B

UV-2E/C

Filter Set # 02 (low [Ca2+])

Data obtained from suppliers.  Omega filters are a product of Omega Optical, Inc.; Chroma filters are a product of Chroma Technology Corp.  *For Nikon filter set, UV is for Ultraviolet Excitation Filter Sets (UV-1A, UV-2A, UV-2B, UV-E/C), V is for Violet Excitation Filter Sets (V-1A, V-2A, BFP), BV is for Blue-Violet Excitation Filter Sets (BV-1A, BV-2A, BV-2B, CFP), B is for Blue Excitation Filter Sets (B-1A, B-2A, B-3A, B-1E, B-2E, B-2E/C), G is for Green Excitation Filter Sets (G-1A, G-1B, G-2A, G-2B, G-2E/C, Cy3 HYQ, TRITC HYQ), Y is for Yellow Excitation Filter Sets (Y-2E/C, Texas Red HYQ), for the Red Excitation Filter Set, there is only Cy5 HYQ;  Zeiss Filter sets are a product of Carl Zeiss Inc. ; If using a microplate reader, attempt to match the excitation and emission filter ranges with the above values, or consult your product company’s literature.  Any other questions, contact MGT Tech-services at techservice@markergene.com.