FionaGreen™ 2X qPCR MasterMix

Product ID: M2954

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Availability: In stock


Easy to use 2X master mix for qPCR applications

FionaGreen™ 2X qPCR MasterMix for quantitative PCR (qPCR) contains FionaGreen™1 dye, our proprietary fluorescent DNA-binding dye that exhibits greater fluorescence enhancement upon binding to double-stranded DNA than many leading dsDNA dyes. FionaGreen™ 2X qPCR MasterMix is a simple-to-use, 2X formulation that includes all components for qPCR with the exception of sample DNA, primers and water.
  • Brighter dsDNA-dependent fluorescence
  • Less PCR inhibition
  • Efficient amplification
  • Lower Ct values
  • Greater linear range
  • Uses the same filters and settings as other dsDNA binding dyes

    Technical Data
    SKU M2954
    Unit Size 1mL
    Emission Wavelength 520
    Detection Method Fluorescence
    Storage Conditions -20C, Protect From Light

    References and Citations


    • Chander Y, Koelbl J, Puckett J, Moser MJ, Klingele AJ, Liles MR, Carrias A, Mead DA, Schoenfeld TW. (2014) "A novel thermostable polymerase for RNA and DNA loop-mediated isothermal amplification (LAMP)." Frontiers in Microbiol. 5: 395.
    • Moser MJ, Chander Y, Mead D, Klingele AJ, Schoenfeld T, Mielke C, Shrago A. (2013) "One-step rapid molecular assay for the detection of Clostridium difficile" Presented at 29th Clinical Virology Symposium, Daytona Beach, FL.
    • Ginzinger DG. (2002) "Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream." Exp Hematol. 30(6):503-12.
    • Heid CA, Stevens J, Livak KJ, Williams PM. (1996) "Real time quantitative PCR." Genome Res. 6(10):986-94.
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