Fluorescein-5-thiosemicarbazide HCl salt

Product ID: M1036

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Cell impermeant fluorescent probe useful for determining protein and peptide topology on the cell surface.

This cell impermeant fluorescent probe is useful for determining protein and peptide topology on the cell surface. It can also be reversibly coupled to aldehydes and ketones to form a Schiff base - which can be further reduced to a generate stable amine derivatives by treatment with reducing agents such as sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNH3). Carboxylic acids of proteins and other water-soluble biopolymers can also be coupled to this fluorescein derivative in aqueous solution using water-soluble carbodiimides such as EDAC.

Technical Data
SKU M1036
CAS Number 76863-28-0
Unit Size 50mg
Absorption 492nm
Emission Wavelength 516
Extinction 85
Detection Method Fluorescence
Molecular Formula C₂₁H₁₆N₃O₅SCl
Molecular Weight 457.89
Soluble In DMF,H2O
Storage Conditions -20C, Desiccated, Protect From Light
Notes Abs/Em in 0.1M Tris, pH=9

References and Citations


  • Qin M, Lee YE, Ray A, Kopelman R. (2014) "Overcoming cancer multidrug resistance by codelivery of doxorubicin and verapamil with hydrogel nanoparticles." Macromol Biosci. 14(8):1106-15.
  • Gattás-Asfura KM, Stabler CL.(2013) "Bioorthogonal layer-by-layer encapsulation of pancreatic islets via hyperbranched polymers." ACS Appl Mater Interfaces. 5(20):9964-74.
  • Qualley DF, Lackey CM, Paterson JP. (2013) "Inositol phosphates compete with nucleic acids for binding to bovine leukemia virus matrix protein: implications for deltaretroviral assembly." Proteins. 81(8):1377-85.
  • Belmont BJ, Niles JC. (2010) "Engineering a direct and inducible protein-RNA interaction to regulate RNA biology." ACS Chem. Biol 5:851-861
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  • "A new strategy for site-specific protein modification: analysis of a Tat peptide-TAR RNA interaction." Tamilarasu N, Zhang J, Hwang S, Rana TM. Bioconjug Chem 12: 135-138 (2001).
  • "Improved Method for Detecting Fluorescent-Labeled Glycoprotein on Sodium Dodecyl Sulfate-Polyacrylamide Gel." Gao X, Liu W-Y, Ruan K. Bioorg Chem 25: 163 (1997).
  • "A fluorescence-labeling method for sequencing small RNA on polyacrylamide gel." Wu TP, Ruan KC, Liu WY. Nucleic Acids Res 24: 3472-3 (1996).
  • "Electrostatic potential of macromolecules measured by pKa shift of a fluorophore. 1. The 3 terminus of 16S RNA." Friedrich K, Woolley P. Eur J Biochem 173: 227-231 (1988).
  • "A simple method for labelling the carbohydrate moieties of antibodies with fluorochromes." Duijndam WA, Wiegant J, Van Duijn P, Haaijman JJ. J Immunol Methods 109: 289-290 (1988).
  • "Use of fluorescein hydrazide and fluorescein thiosemicarbazide reagents for the fluorometric determination of protein carbonyl groups and for the detection of oxidized protein on polyacrylamide gels." Ahn B, Rhee SG, Stadtman ER. Anal Biochem 161: 245-257 (1987).
  • "Direct covalent linkage of fluorescent probes to the plant protoplast surface." Furtula V, Walko RM, Nothnagel EA. Protoplasma 139: 117 (1987).
  • "Distances between 3 ends of ribosomal ribonucleic acids reassembled into Escherichia coli ribosomes." Odom OW Jr, Robbins DJ, Lynch J, Dottavio-Martin D, Kramer G, Hardesty B. Biochemistry 9: 5947-5954 (1980).
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