Fluorescein di-β-D-glucuronide, dimethyl ester

Product ID: M0969



Unit SizePriceQuantity 
2mg
$131.65
  • Buy 5 for $105.31 each and save 21%

Availability: In stock


Description

A cell-permeant β-glucuronidase substrate for live cell and tissue assays of cloned GUS marker gene activity and transfection levels.

Useful in plant molecular biology assays of cloned GUS marker gene activity and transfection. This sensitive, cell-permeant β-glucuronidase substrate can be used for live cell and tissue assays.

Technical Data
SKU M0969
Unit Size 2mg
Absorption 300nm
Emission Wavelength 514
Detection Method Fluorescence
Molecular Formula C₃₄H₃₂O₁₇
Molecular Weight 712.61
Soluble In H2O, DMSO, MeOH
Storage Conditions -20C, Desiccated
Notes Emission of the fluorescein fluorophore (EX 488nm/EM 512nm) released upon beta-glucuronidase enzyme activity.

References and Citations

References:

  • Lorincz M, Roederer M, Diwu Z, Herzenberg LA, Nolan GP. "Enzyme-generated intracellular fluorescence for single-cell reporter gene analysis utilizing Escherichia coli beta-glucuronidase." Cytometry 24, 321-329 (1996).
  • Lorincz MC, Parente MK, Roederer M, Nolan GP, Diwu Z, Martin DI, Herzenberg LA, Wolfe JH. "Single cell analysis and selection of living retrovirus vector-corrected mucopolysaccharidosis VII cells using a fluorescence-activated cell sorting-based assay for mammalian beta-glucuronidase enzymatic activity." J Biol Chem 274, 657-665 (1999).
  • Naleway, JJ., in "GUS Protocols: Using the GUS Gene as a Reporter of Gene Expression", S.R.Gallagher, Ed., Acad. Press, 61-76 (1992).
  • Kwan DH, Chen HM, Ratananikom K, Hancock SM, Watanabe Y, Kongsaeree PT, Samuels AL, Withers SG (2011) "Self-Immobilizing Fluorogenic Imaging Agents of Enzyme Activity" Angewandte Chemie International Edition 50(1): 300–303.
  • Maathuis FJM, May ST, Graham NS, Bowen HC, Jelitto TC, Trimmer P, Bennett MJ, Sanders D, White PJ (1998) "Cell marking in Arabidopsis thaliana and its application to patch–clamp studies." The Plant Journal 15(6): 843-51.
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