Fluorescein diacetate (FDA)

Product ID: M0060

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FDA is one of the most widely-used green fluorogenic substrates for esterase activity. FDA is also a common viability stain for plant cells, protoplasts and tissues.

Upon esterase cleavage of this highly purified probe, fluorescein is released producing a green fluorescence that can be read using standard FITC filter sets.

Technical Data
SKU M0060
CAS Number 596-09-8
Unit Size 1g
Absorption 290nm
Detection Method Fluorescence
Molecular Formula C₂₄H₁₆O₇
Molecular Weight 416.39
Soluble In DMSO
Storage Conditions -20C, Desiccated
Notes Converted to a fluorescent product with pH-dependent spectra similar to 5(6) carboxyfluorescein Product M0122 , (Abs = 492nm, E = 78K, Em = 517nm) after acetate (esterase) hydrolysis. Useful as a cell viability probe in cell culture. See references below for applications.

References and Citations


  • "Analysis of enzyme kinetics in individual living cells utilizing fluorescence intensity and polarization measurements." Deutsch M, Kaufman M, Shapiro H, Zurgil N. Cytometry 39(1): 36-44 (2000).
  • "Probing the physiological state of phytoplankton at the single-cell level." F.J. Jochem. Sci Mar 64: 183 (2000).
  • "Comparative analysis of apoptosis in HL60 detected by annexin-V and fluorescein-diacetate." Bartkowiak D, Hogner S, Baust H, Nothdurft W, Rottinger EM. Cytometry 37(3): 191-196 (1999).
  • "Flow cytometric characteristics of sperm cells isolated from pollen of Zea mays L. Zhang G., et al. Plant Physiol. 99: 54 (1992).
  • "Staining for enzymatic activity after gel electrophoresis." Gersten DM, Gabriel O. Anal. Biochem. 203(2): 181-186 (1992).
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