Fluorescein diphosphate, tetraammonium salt

Product ID: M1034



Unit SizePriceQuantity 
5mg
$269.36
  • Buy 5 for $215.49 each and save 20%

Availability: In stock


Description

Ultrasensitive fluorogenic substrate for alkaline phosphatase analyses.

This alkaline phosphatase substrate is colorless and non-fluorescent prior to enzyme hydrolysis, making it one of the most sensitive substrates for use in coupled assay systems. The green fluorescent dye, fluorescein, is produced upon activity, with EX/EM at 490 nm/520 nm respectively.

Technical Data
SKU M1034
CAS Number 217305-49-2
Unit Size 5mg
Absorption 488nm
Emission Wavelength 512
Detection Method Fluorescence
Molecular Formula C₂₀H₂₆N₄O₁₁P₂
Molecular Weight 560.4
Soluble In H2O, DMSO
Storage Conditions -20C, Desiccated
Notes Abs/Em of fluorescein upon alkaline phosphatase activity.

Other Products You Might Like
MarkerGene™ Fluorescent Alkaline Phosphatase Assay Kit

MarkerGene™ Fluorescent Alkaline Phosphatase Assay Kit

$304.25
     

References and Citations

References:

  • "Measuring the specific activity of the CD45 protein tyrosine phosphatase." Rider DA, Young SP. J Immunol Methods 277: 127-134 (2003).
  • "Development of fluorescence-based selective assays for serine/threonine and tyrosine phosphatases." Pastula C, Johnson I, Beechem JM, Patton WF. Comb Chem High Throughput Screen 6: 341-346 (2003).
  • "Development of a method for evaluating protein tyrosine phosphatase CD45 inhibitors using jurkat cell membrane." Waddleton D, Ramachandran C, Wang Q. Anal Biochem 285: 58-63 (2000).
  • "3,6-Fluorescein diphosphate: a sensitive fluorogenic and chromogenic substrate for protein tyrosine phosphatases." Huang Z, Wang Q, Ly HD, Gorvindarajan A, Scheigetz J, Zamboni R, Desmarais S, Ramachandran C. J Biomol Screen 4(6): 327-334 (1999).
  • "Fluorescein monophosphates as fluorogenic substrates for protein tyrosine phosphatases." Wang Q, Scheigetz J, Gilbert M, Snider J, Ramachandran C. Biochim Biophys Acta 1431: 14-23 (1999).
  • "Fluorogenic substrates for beta-D-galactosidases and phosphatases derived from fluorescein (3,6-dihydroxyfluoran) and its monomethyl ether." Rotman B, Zderic JA, Edelstein M. Proc Natl Acad Sci USA 50: 1 (1963).
>Show more
>Show less

Click here for Newsletter articles about this product


Technical Support

Question about this product? Ask a Scientist!

We pride ourselves on the high quality of our products and want you to get the best possible results from your assays. If you have any questions about this product or need help optimizing your protocol check out the product FAQs below or ask your own question and one of our expert scientists will get back to you asap:


There are currently no frequently asked questions for this product, click the button above to ask a question