Fluorescein diphosphate, tetraammonium salt

Product ID: M1034

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  • Buy 5 for $215.49 each and save 20%

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Ultrasensitive fluorogenic substrate for alkaline phosphatase analyses.

This alkaline phosphatase substrate is colorless and non-fluorescent prior to enzyme hydrolysis, making it one of the most sensitive substrates for use in coupled assay systems. The green fluorescent dye, fluorescein, is produced upon activity, with EX/EM at 490 nm/520 nm respectively.

Technical Data
SKU M1034
CAS Number 217305-49-2
Unit Size 5mg
Absorption 488nm
Emission Wavelength 512
Detection Method Fluorescence
Molecular Formula C₂₀H₂₆N₄O₁₁P₂
Molecular Weight 560.4
Soluble In H2O, DMSO
Storage Conditions -20C, Desiccated
Notes Abs/Em of fluorescein upon alkaline phosphatase activity.

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References and Citations


  • "Measuring the specific activity of the CD45 protein tyrosine phosphatase." Rider DA, Young SP. J Immunol Methods 277: 127-134 (2003).
  • "Development of fluorescence-based selective assays for serine/threonine and tyrosine phosphatases." Pastula C, Johnson I, Beechem JM, Patton WF. Comb Chem High Throughput Screen 6: 341-346 (2003).
  • "Development of a method for evaluating protein tyrosine phosphatase CD45 inhibitors using jurkat cell membrane." Waddleton D, Ramachandran C, Wang Q. Anal Biochem 285: 58-63 (2000).
  • "3,6-Fluorescein diphosphate: a sensitive fluorogenic and chromogenic substrate for protein tyrosine phosphatases." Huang Z, Wang Q, Ly HD, Gorvindarajan A, Scheigetz J, Zamboni R, Desmarais S, Ramachandran C. J Biomol Screen 4(6): 327-334 (1999).
  • "Fluorescein monophosphates as fluorogenic substrates for protein tyrosine phosphatases." Wang Q, Scheigetz J, Gilbert M, Snider J, Ramachandran C. Biochim Biophys Acta 1431: 14-23 (1999).
  • "Fluorogenic substrates for beta-D-galactosidases and phosphatases derived from fluorescein (3,6-dihydroxyfluoran) and its monomethyl ether." Rotman B, Zderic JA, Edelstein M. Proc Natl Acad Sci USA 50: 1 (1963).
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