Product ID: M0204

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Widely used green fluorescent dye for cell staining.

Fluorescein is a widely used green fluorescent fluorophore for use in cell labeling studies. Fluorescein is a fluorophore commonly used in microscopy, as a type of dye laser as the gain medium, as a stain in forensics and serology to detect latent blood stains, and as a standard for many fluorescence based enzyme assays. Fluorescein has an absorption maximum at 494 nm and emission maximum of 521 nm (in water). Several major derivatives of fluorescein are fluorescein isothiocyanate (FITC) and 6-FAM phosphoramidite used in oligonucleotide labeling and sequencing. Fluorescein has an isosbestic point (a point with equal absorption at all pH values) of 460 nm. Fluorescein is also known as a color additive (D&C Yellow no. 7) with the more water soluble disodium salt form of fluorescein is known as uranine (D&C Yellow no. 8).

Technical Data
SKU M0204
CAS Number 7/5/2321
Unit Size 100mg
Absorption 490nm
Emission Wavelength 514
Extinction 90
Detection Method Fluorescence
Molecular Formula C₂₀H₁₂O₅
Molecular Weight 332.3
Soluble In MeOH, DMSO, H2O (pH>8)
Storage Conditions Room Temperature, Desiccated, Protect From Light

References and Citations


  • "Spectral properties of the prototropic forms of fluorescein in aqueous solution." Klonis N, Sawyer WH. J Fluorescence 6: 147 (1996).
  • "Photobleaching kinetics of fluorescein in quantitative fluorescence microscopy." Song L, Hennink EJ, Young IT, Tanke HJ. Biophys. J 68(6): 2588-2600 (1995).
  • "Absorption and fluorescence properties of fluorescein." Sjöback R, Nygren J, Kubista M. Spectrochim. Acta. 51: 7 (1995).
  • "Determination of the absolute quantum yield of fluorescent solutions." Weber G, Teale FWJ. Trans. Faraday Soc. 53: 646 (1957).
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