Fluorescein mono-beta-D-N-acetylglucosamine

Product ID: M1485

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Fluorogenic substrate for hexosaminidase enzymes. Has been used for enzymatic characterization of O-GlcNAcase isoforms.

This fluorogenic substrate has been used for enzymatic characterization of O-GlcNAcase isoforms producing the fluorophore fluorescein upon enzyme activity, with a green emission spectrum (~520 nm).

Technical Data
SKU M1485
CAS Number 148806-95-5
Unit Size 5mg
Alternative Names Fluorescein mono-(N-acetyl-β-D-glucosaminide), Fluorescein mono-β-D-(2-deoxy-2-N-Acetyl)Glucopyranoside, FL-GlcNAc
Absorption 452nm
Emission Wavelength 518
Detection Method Fluorescence
Molecular Formula C₂₈H₂₅NO₁₀
Molecular Weight 535.5
Soluble In H2O, DMSO, EtOH, DMF
Storage Conditions -20C, Desiccated

References and Citations


  • YU H, Liu-bujalski L (2016) "Glycosidase Inhibitors" US Patent Application 20160031871
  • Tanaka Y, Okuda S, Sawai A, Suzuki S. (2012) "Development of a N-acetyl-β-D-glucosaminidase (NAG) assay on a centrifugal lab-on-a-compact-disc (Lab-CD) platform." Anal Sci. 28(1):33-8.
  • Kim EJ, Kang DO, Love DC, Hanover JA, (2006) "Enzymatic characterization of O-GlcNAcase isoforms using a fluorogenic GlcNAc substrate." Carbohydrate Research 341(8):971-982.
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  • Kasai K, Uchida R, Yamaji N, (1993) "Glycosides having chromophores as substrates for sensitive enzyme analysis. IV. Synthesis of N-acetyl-.beta.-D-glucosaminides of fluorescein derivatives and their application to the rate-assay of N-acetylO-GlcNAc-β-D-glucosaminidase." Chemical & Pharmaceutical Bulletin 41(2): 314-18.
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