MarkerGene™ Fluorescent Alkaline Phosphatase Assay Kit

Product ID: M1222

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  • Buy 5 for $243.40 each and save 21%

Availability: In stock


Allows fast and easy measurement of alkaline phosphatase activity in vitro, in cell preparations or in vivo using the substrate fluorescein diphosphate.

Phosphatases utilize a variety of phosphate and polyphosphate esters as substrates, membrane structural components, and energy reservoirs. Simple phosphatases, such as alkaline and acid phosphatase, hydrolyze phosphate monoesters to an alcohol and inorganic phosphate. Alkaline phosphatase plays an important role in many biochemical regulatory pathways, including a possible role in cell differentiation. Alkaline phosphatase activity can be monitored using the fluorogenic substrate, Fluorescein diphosphate, tetraammonium salt (FDP) Product M1034 contained in this kit. Alkaline phosphatase catalyzes a two-step hydrolysis of FDP, releasing the highly fluorescent compound, fluorescein (Product M0204) ,and activity measurements are easily obtained either in vitro, in cell preparations, or in vivo. The kit contains enough substrate for numerous assays and control experiments, and also contains reference standards and a detailed protocol for use. See the references below for more information and applications.

Technical Data
SKU M1222
Unit Size 1kit
Detection Method Fluorescence

References and Citations


  • Ali AT, Penny CB, Paiker JE, van Niekerk C, Smit A, Ferris WF, Crowther NJ (2005). "Alkaline Phosphatase is involved in the control of adipogenesis in the murine preadipocyte cell line, 3T3-L1." Clin Chim Acta 354(1-2): 101-9.
  • Rider DA, Young SP. (2003) "Measuring the specific activity of the CD45 protein tyrosine phosphatase." J Immunol Methods 277: 127-134.
  • Pastula C, Johnson I, Beechem JM, Patton WF (2003). "Development of fluorescence-based selective assays for serine/threonine and tyrosine phosphatases." Comb Chem High Throughput Screen 6: 341-346.
  • Wang Q, Scheigetz J, Roy B, Ramachandran C, Gresser MJ (2002). "Novel caged fluorescein diphosphates as photoactivatable substrates for protein tyrosine phosphatases." Biochim Biophys Acta 1601(1): 19-28.
  • Waddleton D, Ramachandran C, Wang Q. (2000). "Development of a method for evaluating protein tyrosine phosphatase CD45 inhibitors using jurkat cell membrane." Anal Biochem 285: 58-63.
  • Huang Z, Wang Q, Ly HD, Gorvindarajan A, Scheigetz J, Zamboni R, Desmarais S, Ramachandran C. (1999). "3,6-Fluorescein diphosphate: a sensitive fluorogenic and chromogenic substrate for protein tyrosine phosphatases." J Biomol Screen 4(6): 327-334.
  • Wang Q, Scheigetz J, Gilbert M, Snider J, Ramachandran C (1999) "Fluorescein monophosphates as fluorogenic substrates for protein tyrosine phosphatases." Biochim Biophys Acta 1431: 14-23.
  • Rotman B, Zderic JA, Edelstein M (1963). "Fluorogenic substrates for beta-D-galactosidases and phosphatases derived from fluorescein (3,6-dihydroxyfluoran) and its monomethyl ether." Proc Natl Acad Sci USA 50: 1.
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