MarkerGene™ Fluorescent Bacterial Detection and Quantification Kit

Product ID: M1460



Unit SizePriceQuantity 
1 kit
$272.39
  • Buy 5 for $217.91 each and save 21%

Availability: In stock


Description

Employs the highly sensitive substrate FDG, M0250,
a di-β-galactoside conjugate of the dye fluorescein, found to more effectively detect E. Coli and other coliforms through both basal and induced β-galactosidase activity.



Employs the highly sensitive substrate FDG, M0250, a di-β-galactoside conjugate of the dye fluorescein, found to more effectively detect E. Coli and other coliforms through both basal and induced β-galactosidase activity than any other fluorogenic substrate.

Technical Data
SKU M1460
Unit Size 1 kit
Absorption 485nm
Emission Wavelength 520
Detection Method Fluorescence

References and Citations

References:

  • Favier, C.; Neut, C.; Mizon, C.; Cortot, A.; Colombel, J.-F.; Mizon, J. “Differentiation and identification of human fecal anaerobic bacteria producing β-galactosidase (a new methodology).” Journal of Microbiological Methods (1996), 27(1), 25-31.
  • F. Russo-Marie, Roederer, M. Sager, B., Herzenberg, L., “β-Galactosidase Activity in Single Differentiating Bacterial Cells.” Proc. Natl. Acad. Sci. USA 90:8194 (1993).
  • Nelis, H.; Van Poucke, S. “Enzymatic detection of coliforms and Escherichia coli within 4 hours.” Water, Air, and Soil Pollution (2000), 123(1-4): 43-52.
  • Rowland B; Purkayastha A; Monserrat C; Casart Y; Takiff H; McDonough KA (1999) “Fluorescence-based detection of lacZ reporter gene expression in intact and viable bacteria including Mycobacterium species” FEMS Microbiol. Lett. 179(2): 317-25.
  • Kevin L. Griffith and Richard E. Wolf, Jr. (2002) “Measuring β-Galactosidase Activity in Bacteria: Cell Growth, Permeabilization, and Enzyme Assays in 96-Well Arrays” Biochemical and Biophysical Research Communications 290: 397‾402.
  • Miller, J. H. (1972) “Experiments in Molecular Genetics”, Cold Spring Harbor Laboratory Press, Cold Spring Harbor
  • Ross, R. D. , Joneckis, C. C., Ordonez, J. V., Sisk, A. M., Wu, R. K., Hamburger, A. W., and Nora, R. E. (1989). “Estimation of cell survival by flow cytometric quantification of fluorescein diacetate/propidium iodide viable cell number.” Cancer Research. 49: 3776 - 3782.
  • Tao He, Marion G. Priebe, Roel J. Vonk, Gjalt W. Welling. (2005) “Identification of bacteria with β-galactosidase activity in faeces from lactase non-persistent subjects.” FEMS Microbiology Ecology 54: 463-469.
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