MarkerGene™ Fluorescent No-Wash Cell Count Normalization Kit

Product ID: M1745



Unit SizePriceQuantity 
1kit
$134.83
  • Buy 5 for $107.87 each and save 20%

Availability: In stock


Description

This kit provides a simple and ultrasensitive method of measuring cell number for medium to high-throughput cell-based assays.

Medium to high-throughput cell-based assays often require well-to-well normalization in order to quantitate the data generated. In most cases, this normalization requires knowledge of the cell density, or relative number of cells per well. The cell density has been represented by a variety of methods including manual counting using a hemacytometer with or without Trypan blue staining, as well as measuring the DNA content of the cells. In this kit, the ultrasensitive DNA binding bis-benzamide dye is used to stain the nuclear DNA. As opposed to other methods this staining does not require lysis of the cells so can be used in parallel with the main assay with few additional steps.

This assay kit contains all of the reagents for performing up to 1000 assays, i.e. 80 plates using a 24-well format. The assays can also be adapted for use with 96-well, 12-well and 6-well formats. In addition, the kit provides a detailed protocol and references for use.

Technical Data
SKU M1745
Unit Size 1kit
Detection Method Fluorescence

References and Citations

Citations:

  • Hecker L, Logsdon NJ, Kurundkar D, Kurundkar A, Bernard K, Hock T, Meldrum E, Sanders YY, Thannickal VJ. (2014) "Reversal of persistent fibrosis in aging by targeting Nox4-Nrf2 redox imbalance." Sci Transl Med.6(231):231ra47
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References:

  • Richards WL, Song MK, Krutzsch H, Evarts RP, Marsden E and Thorgeirsson SS (1985) "Measurement of cell proliferation in microculture using hoechst 33342 for the rapid semiautomated microfluorimetric determination of chromatin DNA" Experimental Cell Research 159(1): 235-246.
  • Myers MA (1998) "Direct measurement of cell numbers in microtitre plate cultures using the fluorescent dye SYBR green I" Journal of Immunological Methods 212(1): 99-103.
  • Lamboursain L, Jolicoeur M (2005) “Determination of cell concentration in a plant cell suspension using a fluorescence microplate reader.” Plant Cell Rep. 23(10-11): 665-72.
  • Chazotte B.(2011) “Labeling nuclear DNA with Hoechst 33342.” Cold Spring Harbor Protocols.
  • Coleman J, Xiang Y, Pande P, Shen D, Gatica D, Patton WF (2010) “A Live-Cell Fluorescence Microplate Assay Suitable for Monitoring Vacuolation Arising from Drug or Toxic Agent Treatment.” J. Biomol. Screen. 15: 398-405.
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