Fluorescein mono-β-D-galactopyranoside

Product ID: M0064



Unit SizePriceQuantity 
5mg
$164.65
  • Buy 5 for $131.71 each and save 21%

Availability: In stock


Description

Convenient β-galactosidase fluorescent probe. Especially useful for enzymatic analyses where 1st order kinetics are desired.

FMG is especially useful for enzymatic analyses of β-galactosidase where 1st order kinetics are desired. See also our Product M0250.

Technical Data
SKU M0064
CAS Number 102286-67-9
Unit Size 5mg
Absorption 452nm
Emission Wavelength 518
Detection Method Fluorescence
Molecular Formula C₂₆H₂₂O₁₀
Molecular Weight 494.5
Soluble In H2O, MeOH, DMF, DMSO, ETOH
Storage Conditions -20C, Desiccated, Protect From Light

References and Citations

Citations:

  • Jackson K, Khnouf R, Fan ZH. (2014) "Cell-free protein synthesis in microfluidic 96-well plates." Methods Mol Biol. 1118:157-68.
  • Fan ZH, Khnouf R, Mei Q, Jin S. (2010) "A fluid array device for high-throughput protein synthesis." 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Groningen, The Netherlands.
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    References:

    • Hofmann J, Sernetz M, (1983) "A kinetic study on the enzymatic hydrolysis of fluorescein diacetate and fluorescein-di-beta-D-galactopyranoside." Anal. Biochem. 131(1): 180-186
    • Rotman B, Zderic JA, Edelstein M, (1963) "Fluorogenic substrates for beta-D-galactosidases and phosphatases derived from flurescein (3,6-dihydroxyfluoran) and its monomethylether." Proc. Natl. Acad. Sci. USA 50: 1-6.
    • Huang Z, (1991) "Kinetic assay of fluorescein mono-b -D-galactoside hydrolysis by b -galactosidase: a front-face measurement for strongly absorbing fluorogenic substrates." Biochemistry 30(35): 8530-8534.
    • Burke BJ, Regnier FE, (2001) "Electrophoretically mediated microanalysis of b -galactosidase on microchips." Electrophoresis 22(17): 3744-3751.
    • Burke BJ, Regnier FE, (2003),"Stopped-Flow Enzyme Assays on a Chip Using a Microfabricated Mixer." Analytical Chemistry 75(8): 1786-1791.
    • Xu H, Ewing AG, (2004) "A rapid enzyme assay for b -galactosidase using optically gated sample introduction on a microfabricated chip." Analytical and Bioanalytical Chemistry 378(7): 1710-1715.
    • Park SS, Cho SI, Kim MS, Kim YK, Kim BG (2003) "Integration of on-column immobilized enzyme reactor in microchip electrophoresis." Electrophoresis 24(1-2): 200-206.
    • Okhonin V, Liu X, Krylov SN, (2005) "Transverse Diffusion of Laminar Flow Profiles To Produce Capillary Nanoreactors." Analytical Chemistry 77(18): 5925-5929.
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