Enzyme Substrates and Kits for b-Galactosidase

 

b-Galactosidase, an important reporter gene marker encoded by lacZ, is commonly used for monitoring transfection efficiency in mammalian, yeast, and bacterial cells and identifying expression of recombinant fusion genes.1-4 E. coli b-galactosidase has a high turnover rate, hydrolyzing D-galactose from various β-galactosides, which allows for very low level detection in common cell types.  β-Galactosidase substrates can also be used in conjunction with galactosidase-conjugated secondary detection agents in enzyme linked immunosorbent assays (ELISAs) and immunohistochemical techniques.5

 

 

FLUOROGENIC SUBSTRATES AND KITS

 

PRODUCT #

 

PRODUCT NAME

COLOR OF RELEASED PRODUCT (Abs/Em) (nm)

M0064

Fluorescein mono-b-D-Galactopyranoside

Green (490/514)

M0203

Resorufin-b-D-Galactopyranoside (Res-Gal)

Red (571/585)

M0241

4-Methylumbellifery-b-D-Galactopyranside (MUG)

Blue (360/449)

M0250

Fluorescein di-b-D-Galactopyranoside (FDG)

Green (490/514)

M0252

4-Trifluoromethylumbelliferyl-b-D-Galactopyranoside (TFMU-Gal)

Aqua (385/501)

M0257

Carboxyumbelliferyl-b-D-Galactopyranoside (CUG)

Light Blue (330/448)

M0611

3-Carboxyumbelliferyl-b-D-Galactopyranoside-BSA conjugate

Light Blue (330/448)

M1194

2',7'-Dichlorofluorescein di-b-D-Galactopyranoside (DCFDG)

Green (495/529)

M0276

b-Galactosidase Sample Kit

Red (571/585), Green (490/514), Aqua (385/501), Blue (330/448)

M0255

FACS Blue lacZ b-Galactosidase Detection Kit

Light Blue (330/448)

M0259

In vivo lacZ b-Galactosidase Intracellular Detection Kit

Green (490/514)

 

 

CHEMILUMINESCENT SUBSTRATES AND KITS

M0855  

Chemiluminescent lacZ b-Galactosidase Detection Kit

N/A

 

 

REFERENCES:

  1. J Appl Bacteriol 64, 65 (1988)
  2. Appl Environ Microbiol 35, 136 (1978)
  3. Zentralbl Bakteriol 280, 476 (1994)
  4. Infect Immun 61, 5231 (1993)
  5. Mol Cell Probes 9, 145 (1995)