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Products Vectors Enzyme Substrates and Kits Enzyme Inhibitors MGT Detection Kits Cell Regulatory Substrates Special Labeling Reagents Lipids Carbohydrates/Oligosaccharides   Fluorescent Reagents

Enzyme Substrates and Kits for β-Galactosidase β-Galactosidase, an important reporter gene marker encoded by lacZ, is commonly used for monitoring transfection efficiency in mammalian, yeast, and bacterial cells and identifying expression of recombinant fusion genes. E. coli β-galactosidase has a high turnover rate, hydrolyzing D-galactose from various β-galactosides, which allows for very low level detection in common cell types. β-Galactosidase substrates can also be used in conjunction with galactosidase-conjugated secondary detection agents in enzyme linked immunosorbent assays (ELISA) formats and in immunohistochemical techniques.   ID   Name   CAS Unit Size Price Bulk Price (5+) M0252   4-Trifluoromethylumbelliferyl-β-D-galactopyranoside (TFMU-Gal) 117153-55-6 25 mg 101.86 81.49 Blue-green fluorogenic β-galactosidase substrate that has a lower pKa for higher fluorescence in the physiological pH range. M0203   Resorufin β-D-galactopyranoside (Res-Gal) 95079-19-9 10 mg 34.60 27.68 Red fluorogenic substrate for lacZ β-galactosidase activity, intracellularly, or in vitro. M0241   4-Methylumbelliferyl β-D-galactopyranoside (MUG) 6160-78-7 1 g 50.63 40.50 Bright blue fluorogenic β-galactosidase substrate, especially useful for in vitro assays. M1087   D-Luciferin-6-O-β-D-galactopyranoside 131474-38-9 5 mg 150.37 120.30 Analog of D-Luciferin contains a β-galactoside attached at the 6-O-position, and thus is not a substrate for the firefly luciferase enzyme until the galactose is removed e.g., by β-galactosidase activity. As such it represents an ultrasensitive substrate for chemiluminescent measurement of galactosidase activity in homogeneous assays, or in cell lysate samples when the enzyme luciferase is added. M0276   MarkerGene™ β-Galactosidase Sample Kit 1 kit 253.75 203.00 This kit consists of samples of several of our most popular β-galactosidase substrates and their reference fluorophores allowing multiplexed analysis of lacZ β-galactosidase activity at a variety of wavelengths. This kit is perfect for those occasions where the preferred wavelength of detection is under development. M0259   MarkerGene™ in vivo lacZ β-Galactosidase Intracellular Detection Kit 1 kit 190.50 152.40 Allows ultra-sensitive detection of β-galactosidase activity in live mammalian, yeast, bacterial, or plant cells. Especially useful for FACS analysis. M1352   MarkerGene™ β-Galactosidase Staining Kit 1 kit 137.50 110.00 Can be used to monitor and detect enzyme activity in stably or transiently transfected cells or transgenic tissues using the sensitive chromogenic substrate 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal). M1460   MarkerGene™ Fluorescent Bacterial Detection and Quantification Kit 1 kit 210.75 168.60 Employs the highly sensitive substrate fluorescein di-β-D-galactopyranoside (FDG, M0250), a di-β-galactoside conjugate of the dye fluorescein to more effectively detect E. Coli and other coliforms through both basal and induced β-galactosidase activity. M0064   Fluorescein mono-β-D-galactopyranoside (FMGal) 102286-67-9 5 mg 127.38 101.90 Convenient β-galactosidase fluorescent probe. Especially useful for enzymatic analyses where 1st order kinetics are desired. M0250   Fluorescein di-β-D-galactopyranoside (FDG) 17817-20-8 5 mg 86.88 69.50 Ultra-sensitive substrate for lacZ β-galactosidase detection in vivo and in vitro. M0255   Marker Gene™ FACS Fluorescent Blue lacZ β-Galactosidase Detection Kit 1 kit 190.50 152.40 Allows highly sensitive detection of β-galactosidase activity in mammalian, yeast, bacterial, or plant cells, at a shorter wavelength (blue fluorescence) than other kits. This assay is especially amenable to co-staining with a green (fluorescein, GFP) labeling for dual labeling experiments. M1194   2', 7'-Dichlorofluorescein di-β-D-galactopyranoside (DCFDG) N/A 25 mg 79.75 63.80 Highly sensitive fluorescent substrate for measuring galactosidase and galactocerebrosidase activity inside of live cells and lysosomes. M0855   MarkerGene™ Chemiluminescent lacZ β-Galactosidase Detection Kit 1 kit 228.80 183.04 Allows for quantitative measure of β-galactosidase enzyme activity in mammalian, yeast, and bacterial cells through use of a chemiluminescent substrate. M1405   MarkerGene™ Cellular Senescence Microtiterplate Assay Kit 1 Kit 190.50 152.40 Uses the high-sensitivity substrate fluorescein di-β-D-galactopyranoside (FDG) to quantify senescent cells. M0611   3-Carboxyumbelliferyl β-D-galactoside, BSA conjugate N/A 2 mg 82.72 66.18 Useful for detection of extracellular galactosidase activity, from enzyme-linked antibody systems or for endocytosis experiments, measurement of lacY permease activity, or microinjection analyses. M0257   Carboxyumbelliferyl β-D-galactopyranoside (CUG) 64664-99-9 5 mg 108.50 86.80 Blue fluorogenic beta-galactosidase substrate that is well retained intracellularly. The fluorescence spectrum for the released 3-carboxyumbelliferone is pH-dependent.

©2010 Marker Gene Technologies, Inc. Published by Marker Gene Technologies, Inc., The University of Oregon Riverfront Research Park, 1850 Millrace Drive, Eugene, Oregon 97403-1992 USA. All rights reserved. For information on the use or copying of the material contained in this document, please contact us at techservice@markergene.com.