MarkerGene™ β-Glucuronidase (GUS) Reporter Gene Activity Detection Kit

Product ID: M0877



Unit SizePriceQuantity 
1kit
$298.88
  • Buy 5 for $239.11 each and save 20%

Availability: In stock


Description

Allows for quantitative measurement of GUS enzyme activity in transformed plants through use of a fluorogenic substrate.

The β-glucuronidase (GUS) enzyme from E. coli (EC 3.2.1.31) has been well documented to provide desirable characteristics as a marker gene in transformed plants. The GUS reporter gene system has many advantages including stable expression of E. coli GUS enzyme, no interference with normal plant metabolism, and low intrinsic GUS activity in higher plants. The enzyme is also capable of tolerating amino-terminal additions, making it useful for study of plant organelle transport [1,2,3,4]. Various β-glucuronic acid substrates are available for detection of GUS expression, all of which contain the sugar D-glucopyranosiduronic acid attached by glycosidic linkage to a hydroxyl group of a chromogenic, fluorogenic, or other detectable molecule [5]. The most widely used fluorogenic substrate for detection of β-glucuronidase activity in vitro is 4-methylumbelliferyl β-D-glucuronide (4-MUG). Upon hydrolysis by GUS, the fluorochrome 4-methylumbelliferone (7-hydroxy-4-methyl coumarin) is produced along with sugar glucuronic acid [5]. It is used in this fluorescent activity detection test. Please contact our technical services department for further information.

Technical Data
SKU M0877
Unit Size 1kit
Detection Method Fluorescence

References and Citations

Citations:

  • Hesari N, Alum A, Elzein M, Abbaszadegan M. (2016) "A biosensor platform for rapid detection of E. coli in drinking water." Enzyme and Microbiol Tech 83: 22-28. http://dx.doi.org/10.1016/j.enzmictec.2015.11.007
  • Almeyda CV, Raikhy G, Pappu HR. (2015) "Characterization and comparative analysis of promoters from three plant pararetroviruses associated with Dahlia (Dahlia variabilis)." Virus Genes. 51(1):96-104.
  • Li L, Song Y, Wang K, Dong P, Zhang X, Li F, Li Z, Ren M. (2015) "TOR-inhibitor insensitive-1 (TRIN1) regulates cotyledons greening in Arabidopsis." Front Plant Sci. 6:861.
  • Shepherd DN, Dugdale B, Martin DP, Varsani A, Lakay FM, Bezuidenhout ME, Monjane AL, Thomson JA, Dale J, Rybicki EP (2014) "Inducible Resistance to Maize Streak Virus." Lin B, ed. PLoS ONE 9(8):e105932. doi:10.1371/journal.pone.0105932.
  • Chen Y, Yordanov YS, Ma C, Strauss S, Busov VB. (2013) "DR5 as a reporter system to study auxin response in Populus." Plant Cell Rep. 32(3):453-63.
  • Behringer C, Bartsch K, Schaller A. (2011) "Safeners recruit multiple signalling pathways for the orchestrated induction of the cellular xenobiotic detoxification machinery in Arabidopsis." Plant Cell Environ. 34(11):1970-85.
  • Horváth BM, Magyar Z, Zhang Y, Hamburger AW, Bakó L, Visser RG, Bachem CW, Bögre L.(2006) "EBP1 regulates organ size through cell growth and proliferation in plants." EMBO J.25(20):4909-20.
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    References:

    • Gallagher SR. (1992) GUS Protocols: Using the GUS Gene as a Reporter of Gene Expression. Academic Press, Inc.
    • Kosugi S, Ohashi Y, Nakajima K, Arai Y. (1990) “An improved assay for beta-glucuronidase in transformed cells: methanol almost completely suppresses a putative endogenous for beta-glucuronidase activity.” Plant Sci 70: 130-140.
    • Jefferson RA, Kavanagh TA, Bevan MW. (1987) “GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.” EMBO J 6: 3901-3907.
    • Jefferson RA, Burgess SM, Hirsh D. (1986) “beta-Glucuronidase from Escherichia coli as a gene fusion marker.” Proc Natl Acad Sci USA. 83: 8447-8451.
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