in vivo N-AcDEVD-Caspase 3/7 Substrate N

Product ID: M0710

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in vivo caspase 3 substrate for intracellular apoptosis detection.

This new Caspase 3/7 substrate can be used for intracellular apoptosis detection. Cleavage of the specific DEVD peptide releases the highly fluorescent dye rhodol, with strong fluorescence emission at 520-520 nm upon excitation at 490 nm. The C-9 alkyl ether ensures both cell permeability as well as retention inside the cell after activity.

Technical Data
SKU M0710
Unit Size 500μg
Absorption 494nm
Emission Wavelength 521
Detection Method Fluorescence
Molecular Weight 958.04
Soluble In DMF, DMSO
Storage Conditions -20C, Desiccated
Notes Make solution in organic solvents prior to dissolving in media for cellular labeling studies.

References and Citations


  • Guzikowski AP, Naleway JJ, Shipp CT, Shutte RC.(2000) "Synthesis of a macrocyclic rhodamine 110 enzyme substrate as an intracellular probe for caspase 3 activity.” Tett. Lett. 41 4733-4735.
  • Guzikowski AP, Naleway JJ, Shipp CT, Shutte RC. Howard RA, Braden MR. (2000)"Cyclic Peptidase Substrates for Fluorescent Analysis of Caspase 3 Enyzme Activity" Proc. SPIE 3913:54-63.
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