2', 7'-Dichlorofluorescein di-Β-D-lactoside

2', 7'-Dichlorofluorescein di-Β-D-lactoside

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Quick Overview

Highly sensitive fluorescent substrate for measuring Β-lactosidase or galactosidase and glucosidase activity inside of live cells and lysosomes.

2', 7'-Dichlorofluorescein di-Β-D-lactoside

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Details

This substrate releases the highly fluorescent fluorophore 2',7'-dichlorofluorescein (EX: 495nm/EM: 529 nm) at the site of Β-lactosidase or galactosidase and glucosidase activity. Since the pKa of the released fluorophore is significantly lower than comparable fluorophores, it can retain appreciably more fluorescence in the highly acidic environment of the lysosome than other similar fluorophores

Additional Information

SKU M1416
Unit Size 5mg
Molecular Formula C₄₄H₅₀Cl₂O₂₅
Molecular Weight 1049.76
References
  • Miura Y, Kim S, Etchison JR, Ding Y, Hindsgaul O, Freeze HH. (2000) "Aglycone structure influences a-fucosyltransferase III activity using N-acetyllactosamine glycoside acceptors." Glycoconjugate J 16(11):725-730.
  • Minke WE, Roach C, Hol WGJ, Verlinde CLMJ. (1999) "Structure-Based Exploration of the Ganglioside GM1 Binding Sites of Escherichia coli Heat-Labile Enterotoxin and Cholera Toxin for the Discovery of Receptor Antagonists." Biochem 38(18): 5684-5692.
  • Daniels L.B., Glew R.H., Diven W.F., Lee R.E., Radin N.S., (1981) "An improved fluorimetric leukocyte b-glucosidase assay for Gauchers disease." Clin. Chim. Acta 115: 369-375.