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Fluorescent Reagents Fluorescent Reagents
Marker Gene Detection Kits

Marker Gene Technologies provides a variety of convenient kits for marker gene detection and monitoring. Each kit comes complete with reagents, standards, protocols, and references. The staff at Marker Gene Technologies is also available to answer any questions and provide additional information.

  ID   Name   CAS Unit Size Price Bulk Price (5+)
M0972   MarkerGene™ TAMRA Antibody/Protein/Cell Labeling Kit 1 kit 200.97 160.78
Provides reagents and methods to simply and quickly label antibodies or other proteins with the amine-reactive dye 5(6)-carboxytetramethylrhodamine.
M1576   RubyGlowTM Luminescent Cytotoxicity Assay Kit 1 kit 110.00
Most commercially available luciferase kits use green light emitting (562 nm) firefly analogs. The RubyGlowTM luminescent assay kits produce a red light (EM 619 nm) for measurement of cell number, cell proliferation, growth, viability or cytotoxicity based upon the generation of ATP in metabolically active cells. This unique feature enables the possibility of multiplexing. The new kit components involve no radioisotopes or toxic materials and are environmentally safe. Each kit provides enough reagents and a detailed protocol sufficient for 100 reactions in 96-well microplate format.
M1575   RubyGlowTM Luminescent Cell Viability Assay Kit 1 kit 110.00
Most commercially available luciferase kits use green light emitting (562 nm) firefly analogs. The RubyGlowTM luminescent assay kits produce a red light (EM 619 nm) for measurement of cell number, cell proliferation, growth, viability or cytotoxicity based upon the generation of ATP in metabolically active cells. This unique feature enables the possibility of multiplexing. The new kit components involve no radioisotopes or toxic materials and are environmentally safe. Each kit provides enough reagents and a detailed protocol sufficient for 100 reactions in 96-well microplate format.
M1574   RubyGlowTM Luminescent Cell Proliferation Assay Kit 1 kit 110.00
Most commercially available luciferase kits use green light emitting (562 nm) firefly analogs. The RubyGlowTM luminescent assay kits produce a red light (EM 619 nm) for measurement of cell number, cell proliferation, growth, viability or cytotoxicity based upon the generation of ATP in metabolically active cells. This unique feature enables the possibility of multiplexing. The new kit components involve no radioisotopes or toxic materials and are environmentally safe. Each kit provides enough reagents and a detailed protocol sufficient for 100 reactions in 96-well microplate format.
M1475   Marker Gene™ Non-Radioactive Cell Proliferation Assay Kit 1 Kit 235.20 188.16

The MarkerGene™ Non-Radioactive Cell Proliferation Assay Kit uses a common tetrazolium dye MTT ((3-(4,5-imethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) as a method of quickly and accurately measuring cell viability. The assay relies on the reduction by live cells of the tetrazolium salt MTT by intracellular oxidoreductases. It is therefore widely regarded as an accurate indicator of cell redox activity.

M1580   MarkerGene™ Multiple Drug Resistance Microtiterplate Assay Kit 210.00 168.00
This kit contains protocols and reagents for use in the detection of Multiple Drug Resistance within cells.
M0271   MarkerGene™ Membrane Fluidity Kit 1 kit 118.13 94.50
Allows quantitative monitoring of membrane fluidity in cell membranes, micelles, and vesicles through use of a lipophilic pyrene probe.
M1389   MarkerGene™ Cellular Senescence Assay Kit 1 kit 202.65 162.12
Can be used to monitor and detect β-galactosidase activity in senescent cells at pH 6.0 using X-Gal.
M1377   MarkerGene™ LysoLive™ Lysosomal Sulfatase Assay Kit 1 kit 331.80 265.44
Useful for labeling lysosomes in a live-cell format and capable of monitoring lysosomal metabolic activity.
M1376   MarkerGene™ LysoLive™ Lysosomal Phosphatase Assay Kit 1 kit 331.80 265.44
Useful for labeling lysosomes in a live-cell format and capable of monitoring lysosomal metabolic activity.
M1573   RubyGlowTM Luminescent Bacterial Detection Kit 1 kit 110.00
The new RubyGlowTM Luminescent Bacterial Detection Kit can detect bacteria from a number of media or sources with an extremely high sensitivity (low detection limit). In addition, extended incubation (2-4 hours) of samples at 37ºC can significantly lower the detection limit and increase sensitivity using a protocol that can be completed in one day. Data variation is low and confidence is high compared with several current “Add and Read” commercial kits. Due to its stable light emission, we have adapted this kit for use in a microtiter plate format; thus enabling the processing of a large number of samples (such as food or clinical samples) at the same time. This microplate format assay is amenable to high throughput (HTS) applications for analysis of bacterial susceptibility and antibiotic screening. For more information contact us at techservice@markergene.com.
M1214   MarkerGene™ Long Wavelength Fluorescent Lipase Assay Kit 1 kit 266.44 213.15
Allows fast and easy measurement of lipase activity in vitro, in cell preparations or in vivo using the long wavelength substrate resorufin oleate.
M0795   MarkerGene™ Live:Dead /Cytotoxicity Assay Kit 1 kit 200.97 160.78
Allows fast and easy measurement of both living and dead cells by measuring intracellular esterase activity (live cells) and staining of nucleic acids in chromatin (dead cells) in vitro, and in cell culture.
M0626   MarkerGene™ Live Cell Luciferase Assay Kit 1 kit 106.58 85.26
Contains synthetic D-luciferin, the primary substrate of the firefly luciferase light producing system, as well as a luciferase assay buffer
M1045   MarkerGene™ Live Cell Glutathione Transferase Activity Kit 1 kit 160.55 128.44
Provides reagents and methods to simply and quickly measure glutathione transferase activity in live cells, tissues or cell lysate samples
M1049   MarkerGene™ Live Cell Fluorescent Reactive Oxygen Species Detection Kit 1 kit 200.97 160.78
Provides all necessary reagents, buffers and a detailed protocol for the detection of Reactive Oxygen Species (ROS, peroxidase activity) in live cells.
M0794   MarkerGene™ Hydrophobic Protein Analysis Kit 1 kit 228.38 182.70
Allows fast and easy measurement of proteins in complex mixtures using either solution analysis or 1D- or 2D-SDS-PAGE analysis. Both denatured or native conformations of proteins isolated from cell preparations can be analyzed.
M0877   MarkerGene™ β-Glucuronidase (GUS) Reporter Gene Activity Detection Kit 1 kit 242.81 194.25
Allows for quantitative measurement of GUS enzyme activity in transformed plants through use of a fluorogenic substrate.
M1193   MarkerGene™ Gaussia Luciferase Cellular Assay Kit 1 kit 71.61 57.29
Gaussia luciferase is a new reporter gene isolated from the marine copepod Gaussia princeps, and can be expressed in mammalian cells using commercially available reporter plasmids. This luciferase, which does not require ATP, catalyzes the oxidation of the substrate (M0739) in a reaction that emits light (at 470 nm), and has considerable advantages over other reporter genes.
M0276   MarkerGene™ β-Galactosidase Sample Kit 1 kit 266.44 213.15
This kit consists of samples of several of our most popular β-galactosidase substrates and their reference fluorophores allowing multiplexed analysis of lacZ β-galactosidase activity at a variety of wavelengths. This kit is perfect for those occasions where the preferred wavelength of detection is under development.
M0259   MarkerGene™ in vivo lacZ β-Galactosidase Intracellular Detection Kit 1 kit 200.03 160.02
Allows ultra-sensitive detection of β-galactosidase activity in live mammalian, yeast, bacterial, or plant cells. Especially useful for FACS analysis.
M1352   MarkerGene™ β-Galactosidase Staining Kit 1 kit 144.38 115.50
Can be used to monitor and detect enzyme activity in stably or transiently transfected cells or transgenic tissues using the sensitive chromogenic substrate 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal).
M1319   MarkerGene™ Fluorescent Protease Assay Kit 1 kit 170.10 136.08
Allows quick and simple measurement of general protease activity in biological samples.
M0612   MarkerGene™ Fluorescent Lipase Assay Kit [102807-51-2] for substrate reagent 1 kit 266.44 213.15
Allows fast and easy measurement of lipase activity in vitro, in cell preparations or in vivo using the fluorescent fatty acid substrate 1,2-dioleoyl-3-(pyren-1-yl)decanoyl-rac-glycerol M0258.
M1245   MarkerGene™ Fluorescent Cellulase Assay Kit 1 kit 240.19 192.15
Allows fast and easy detection of most cellulases in a microtiter plate based assay format.
M1460   MarkerGene™ Fluorescent Bacterial Detection and Quantification Kit 1 kit 221.29 177.03
Employs the highly sensitive substrate fluorescein di-β-D-galactopyranoside (FDG, M0250), a di-β-galactoside conjugate of the dye fluorescein to more effectively detect E. Coli and other coliforms through both basal and induced β-galactosidase activity.

M1222   MarkerGene™ Fluorescent Alkaline Phosphatase Assay Kit 1 kit 247.17 197.74
Allows fast and easy measurement of alkaline phosphatase activity in vitro, in cell preparations or in vivo using the substrate fluorescein diphosphate.
M0955   MarkerGene™ FITC Antibody Labeling Kit 1 kit 182.70 146.16
Provides reagents and methods to simply and quickly label antibodies or other proteins with the brilliant green dye fluorescein.
M0255   MarkerGene™ FACS Fluorescent Blue lacZ β-Galactosidase Detection Kit 1 kit 200.03 160.02
Allows highly sensitive detection of β-galactosidase activity in mammalian, yeast, bacterial, or plant cells, at a shorter wavelength (blue fluorescence) than other kits. This assay is especially amenable to co-staining with a green (fluorescein, GFP) labeling for dual labeling experiments.
M0855   MarkerGene™ Chemiluminescent lacZ β-Galactosidase Detection Kit 1 kit 240.24 192.19
Allows for quantitative measure of β-galactosidase enzyme activity in mammalian, yeast, and bacterial cells through use of a chemiluminescent substrate.
M1405   MarkerGene™ Cellular Senescence Microtiterplate Assay Kit 1 Kit 200.03 160.02
Uses the high-sensitivity substrate fluorescein di-β-D-galactopyranoside (FDG) to quantify senescent cells.
M0272   MarkerGene™ Carbohydrate Analysis Detection Kit 1 kit 209.48 167.58
Allows the user to quickly estimate and/or compare the composition of carbohydrates obtained from glycoproteins.
M1138   MarkerGene™ Biotin-X Antibody/Protein Labeling Kit 1 kit 233.10 186.48
For efficient biotinylation of antibodies and other proteins. The long spacer between the biotin and the reactive group in biotin-X succinimidyl ester enhances the ability of the conjugated biotin to interact with avidin and streptavidin.
M1356   MarkerGene™ Biotin Detection and Quantitation Kit 1 kit 120.75 96.60
Allows the measurement of the molar ratio of biotin to protein in biotin-protein conjugates, biotin-DNA molar ratios in biotinylated DNA samples, or quantitation of biotin concentrations in solution. The kit can be used to determine biotin concentrations down to 1 uM concentrations. The assay can be performed in a microtiterplate format.
M1745   MarkerGene™ Fluorescent No-Wash Cell Count Normalization Kit 115.00 92.00
Medium to high-throughput cell-based assays often require well-to-well normalization in order to quantitate the data generated. In most cases, this normalization requires knowledge of the cell density, or relative number of cells per well. The cell density has been represented by a variety of methods including manual counting using a hemacytometer with or without Trypan blue staining, as well as measuring the DNA content of the cells. In this kit, the ultrasensitive DNA binding bis-benzamide dye is used to stain the nuclear DNA. As opposed to other methods this staining does not require lysis of the cells so can be used in parallel with the main assay with few additional steps.
M1585   MarkerGene™ Total Protein Determination Kit 85.00
The ability to quantify the amount of protein in a sample is vital to almost all aspects of research. This quantification should be both quick and easy to perform. To this end the MarkerGene™ Total Protein Determination Kit which utilizes the principles of the Bradford Assay for protein quantification is very useful, allowing the amount of protein in a sample to be deduced in a very short time.

©2012 Marker Gene Technologies, Inc. Published by Marker Gene Technologies, Inc., The University of Oregon Riverfront Research Park, 1850 Millrace Drive, Eugene, Oregon 97403-1992 USA. All rights reserved. For information on the use or copying of the material contained in this document, please contact us at techservice@markergene.com.