| Product ID |
Product Name |
Description |
| M0255 |
Marker GeneTM FACS Fluorescent Blue lacZ ß-Galactosidase Detection Kit |
The MarkerGeneTM FACS lacZ ß-Galactosidase Detection Kit allows highly sensitive detection of ß-galactosidase activity in mammalian, yeast, bacterial, or plant cells, at a shorter wavelength (blue fluorescence) than other kits. This assay is especially amenable to co-staining with a green (fluorescein, GFP) labeling for dual labeling experiments. |
| M0259 |
MarkerGeneTM in vivo lacZ ß-Galactosidase Intracellular Detection Kit |
The MarkerGeneTM in vivo lacZ ß-Galactosidase Intracellular Detection Kit allows ultra-sensitive detection of ß-galactosidase activity in live mammalian, yeast, bacterial, or plant cells. Especially useful for FACS analysis. |
| M0271 |
MarkerGeneTM Membrane Fluidity Kit |
The Membrane Fluidity Kit allows quantitative monitoring of membrane fluidity in cell membranes, micelles, and vesicles through use of a lipophilic pyrene probe. |
| M0272 |
MarkerGeneTM Carbohydrate Analysis Detection Kit |
The Carbohydrate Analysis/Detection Kit allows the user to quickly estimate and/or compare the composition of carbohydrates obtained from glycoproteins. |
| M0276 |
MarkerGeneTM ß-Galactosidase Sample Kit |
This kit consists of samples of several of our most popular galactosidase substrates and their reference fluorophores allowing multiplexed analysis of lacZ β-Galactosidase activity at a variety of wavelengths. This kit is perfect for those occasions where the preferred wavelength of detection is under development. |
| M0612 |
MarkerGeneTM Fluorescent Lipase Assay Kit |
The Lipase Assay Kit allows fast and easy measurement of lipase activity in vitro, in cell preparations or in vivo using the fluorescent fatty acid substrate 1,2-dioleoyl-3-pyrenyldecanoyl-rac-glycerol (Product M0258). |
| M0626 |
MarkerGeneTM Live Cell Luciferase Assay Kit |
This kit contains synthetic D-luciferin, the primary substrate of the firefly luciferase light producing system, as well as a luciferase assay buffer (10 mL, for a 1 mM reagent concentration), and other reagents, enough to perform 1000 assays using a microtiterplate format (100 uL reaction volume, 0.1 mM final reaction concentration). 
The synthetic beetle luciferin substrate contained in the kit is also available separately as a lyophilized powder D-Luciferin, Product M0237. Consult the Product information sheet (above) for more details on the assay protocol.
|
| M0794 |
MarkerGeneTM Hydrophobic Protein Analysis Kit |
The Hydrophobic Protein Analysis Kit allows fast and easy measurement of proteins in complex mixtures using either solution analysis or 1D- or 2D-SDS-PAGE analysis. Both denatured or native conformations of proteins isolated from cell preparations can be analyzed. The kit uses the environment-sensitive fluorescent probe 2-p-toluidinylnapthalene-6-sulfonic acid (TNS) (Product M0704). TNS is practically non-fluorescent in water or buffer solutions, but exhibits a strong fluorescence enhancement when bound to hydrophobic regions of proteins. By incorporating the TNS dye in standard gel-electrophoresis procedures, hydrophobic proteins (typically from membranes) become fluorescent. Try this new kit with your proteomic analyses today! |
| M0795 |
MarkerGeneTM Live:Dead Assay Kit |
Our Live:Dead Assay Kit allows fast and easy measurement of both living and dead cells by measuring intracellular esterase activity (live cells) and staining of nucleic acids in chromatin (dead cells) in vitro, in cell culture using the fluorescent substrate 5(6)-carboxyfluorescein diacetate (Product M0011) and the membrane impermeant DNA stain, propidium iodide(Product M0793). The assay is amenable to high-throughput assays (fluorescence microtiterplate systems), FACS analysis and for staining of live mammalian, bacterial or yeast cells in culture. Please see the kit Product Information Sheet (Adobe Acrobat .pdf file at the top of this page) or the references below for more information about the use of this kit. |
| M0855 |
MarkerGeneTM Chemiluminescent lacZ ß-Galactosidase Detection Kit |
The Chemiluminescent lacZ ß-Galactosidase Detection Kit allows for quantitative measure of galactosidase enzyme activity in mammalian, yeast, and bacterial cells through use of a chemiluminescent substrate. |
| M0877 |
MarkerGeneTM ß-Glucuronidase (GUS) Reporter Gene Activity Detection Kit |
This is one of our fluorescent stains for live plant cells. The ß-Glucuronidase (GUS) Reporter Gene Activity Detection Kit allows for quantitative measure of GUS enzyme activity in transformed plants through use of a fluorogenic substrate. |
| M0955 |
MarkerGeneTM FITC Antibody Labeling Kit |
This NEW* kit provides reagents and methods to simply and quickly label antibodies or other proteins with the brilliant green dye fluorescein. |
| M0972 |
MarkerGeneTM TAMRA Antibody/Protein/Cell Labeling Kit |
This NEW* kit provides reagents and methods to simply and quickly label antibodies or other proteins with the amine-reactive dye 5(6)-carboxytetramethylrhodamine. |
| M1045 |
MarkerGeneTM Live Cell Glutathione Transferase Activity Kit |
This kit provides reagents and methods to simply and quickly measure glutathione transferase activity in live cells, tissues or cell lysate samples |
| M1049 |
MarkerGeneTM Live Cell Fluorescent Reactive Oxygen Species Detection Kit |
The Live Cell Fluorescent Reactive Oxygen Species Detection Kit provides all necessary reagents, buffers and a detailed protocol for the detection of Reacive Oxygen Species (ROS, peroxidase activity) in live cells. The assay utilizes the cell permeable reagent, 2,7-dichlorofluorescin diacetate (Product M0807) , a reliable fluorogenic marker for ROS detection. Upon enzyme activity, the highly fluorescent dye, 2,7-dichlorofluorescein is produced, with EX: 495nm and EM: 529 nm. |
| M1138 |
MarkerGeneTM Biotin-X Antibody/Protein Labeling Kit |
For efficient biotinylation of antibodies and other proteins.The kit contains biotin-X succinimidyl ester, which reacts with primary amines of proteins, antibodies or other biomolecules to form stable biotin conjugates. The long spacer between the biotin and the reactive group in biotin-X succinimidyl ester enhances the ability of the conjugated biotin to interact with avidin and streptavidin. Also included in the kit are a gel filtration spin-column to purify the biotinylated protein from excess biotin, reagents for quantitating the degree of biotinylation and a control. This kit contains sufficient reagents for at least five biotinylation reactions of 1–5 mg each. |
| M1193 |
MarkerGeneTM Gaussia Luciferase Cellular Assay Kit |
The MarkerGeneTM Gaussia Luciferase Cellular Assay Kit contains enough reagents for 100 assays using a microtiterplate format and includes buffers, solvents and a detailed protocol for use. |
| M1214 |
MarkerGeneTM Long Wavelength Fluorescent Lipase Assay Kit |
The Lipase Assay Kit allows fast and easy measurement of lipase activity in vitro, in cell preparations or in vivo using the substrate resorufin oleate. |
| M1222 |
MarkerGeneTMFluorescent Alkaline Phosphatase Assay Kit |
The Fluorescent Alkaline Phosphatase Assay Kit allows fast and easy measurement of Alkaline Phosphatase activity in vitro, in cell preparations or in vivo using the substrate fluorescein diphosphate. |
| M1245 |
MarkerGeneTM Fluorescent Cellulase Assay Kit |
The Fluorescent Cellulase Assay Kit allows fast and easy detection of most cellulases in a microtiter plate based assay format. |
| M1319 |
MarkerGeneTM Fluorescent Protease Assay Kit |
The Fluorescent Protease Assay Kit allows quick and simple measurement of general protease activity in biological samples. |
| M1352 |
MarkerGeneTM ß-Galactosidase Staining Kit |
The E. coli lacZ gene is
among the most widely used marker genes for mammalian, yeast and bacterial transfection studies due in part to its resistance to intracellular proteolytic degradation. The lacZ gene encodes for the ß-Galactosidase (ß-Gal) enzyme that catalyzes the hydrolysis of a wide range of ß-galactosides. The MarkerGeneTM ß-Galactosidase Staining Kit can be used to monitor and detect enzyme activity in stably or transiently transfected
cells or transgenic tissues using the sensitive chromogenic substrate 5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside
(X-Gal). When ß-Gal cleaves the glycosidic linkage in X-Gal, a soluble, colorless indoxyl derivative is produced. which quickly dimerizes and becomes oxidized to produce a bright blue indigo dye precipitate at the site of ß-Gal activity. The dimerization
and oxidation reaction is facilitated, in this kit, by a developer buffer solution containing ferric and ferrous ions. Both transfection efficiency and comparative expression levels can be determined by examining and counting the number of blue cells in the total cell population. |
| M1356 |
MarkerGeneTM Biotin Detection and Quantitation Kit |
The MarkerGeneTM Biotin Detection and Quantitation Kit allows the measurement of the molar ratio of biotin to protein in biotin-protein conjugates, biotin-DNA molar ratios in biotinylated DNA samples, or quantitation of biotin concentrations in solution. The assay employs the sensitive chromogenic reagent 4'-hydroxyazobenzene-2-carboxylic acid (HABA) that exhibits a UV spectral absorbance at 500 nm when bound to avidin. Because biotin easily displaces HABA from HABA/Avidin complexes, the resulting decrease in absorption at 500 nm can be used to sensitively measure biotin concentrations. The kit can be used to determine biotin concentrations down to 1 uM concentrations. The assay can be performed in a microtiterplate format. |
| M1376 |
MarkerGeneTM LysoLiveTM Lysosomal Phosphatase Assay Kit |
Lysosomes are acidic cytoplasmic organelles that are present in all nucleated mammalian cells. Lysosomes have been found to be involved in a variety of cellular processes including repair of the plasma membrane, defense against pathogens, cholesterol homeostasis, bone remodeling, metabolism, apoptosis, and cell signaling. Defects in lysosomal enzyme activity have been associated with a variety of diseases including Parkinson's, Tay-Sachs, Sandhoff, Krabbe, Wolman, and Gaucher syndromes. Marker Gene has developed lysosomal staining compounds that are useful for labeling lysosomes in a live-cell format and are capable of monitoring lysosomal metabolic activity. These new targeted substrates are based upon fluorescent probes that have a low pKa value for optimum fluorescence at the lower physiological pH values found in the lysosomes as well as targeting groups to direct their accumulation to the lysosomes using a live-cell staining format. |
| M1377 |
MarkerGeneTM LysoLiveTM Lysosomal Sulfatase Assay Kit |
Lysosomes are acidic cytoplasmic organelles that are present in all nucleated mammalian cells. Lysosomes have been found to be involved in a variety of cellular processes including repair of the plasma membrane, defense against pathogens, cholesterol homeostasis, bone remodeling, metabolism, apoptosis, and cell signaling. Defects in lysosomal enzyme activity have been associated with a variety of diseases including Parkinson's, Tay-Sachs, Sandhoff, Krabbe, Wolman, and Gaucher syndromes. Marker Gene has developed lysosomal staining compounds that are useful for labeling lysosomes in a live-cell format and are capable of monitoring lysosomal metabolic activity. These new targeted substrates are based upon fluorescent probes that have a low pKa value for optimum fluorescence at the lower physiological pH values found in the lysosomes as well as targeting groups to direct their accumulation to the lysosomes using a live-cell staining format. |
| M1389 |
MarkerGeneTM Cellular Senescence Assay Kit |
Many primary cell types, particularly fibroblast cells, have a limited capacity to reproduce in cell culture. Even normal cells derived from fetal, embryonic or newborn tissue, typically undergo between 40 and 60 cell divisions, but then often stop dividing becoming senescent. When cells become senescent changes in morphology and gene expression patterns occur. In addition, senescence is also accompanied by increases in acidic senescence-associated ß-galactosidase (SA-ß-Gal). This activity can be monitored using the substrate 5-bromo-4chloror-3-indolyl-ß-galactopyranoside (X-Gal) at pH 6 and is a useful enzymatic marker of cellular senescence (SA-ß-Gal). While cells entering senescence show this enzymatic activity, immortalized cell types, including tumor or other quiescent cells, are not stained under these conditions. The implications of these methods in such diverse areas as age-related pathology research, tumor analysis and tissue culture maintenance is significant. The MarkerGeneTM Cell Senescence Assay Kit can be used to monitor and detect ß-galactosidase activity in senescent cells at pH 6.0 using X-Gal. When ß-Gal cleaves the glycosidic linkage in X-Gal, a soluble, colorless indoxyl derivative is produced, which quickly dimerizes and becomes oxidized to produce a bright blue indigo dye precipitate at the site of ß-Gal activity. The dimerization and oxidation reaction is facilitated, in this kit, by a developer buffer solution containing ferric and ferrous ions. Senescent cells can be determined by examining the number of blue cells in the total cell population. The kit provides enough reagents and standards for up to 100 assays, using a microtiterplate method, and includes a detailed protocol for use in cultured mammalian cells, as well as yeast and bacterial samples. |
