| Product ID |
Product Name |
Description |
| M0013 |
5(6)-Carboxyfluorescein diacetate, NHS ester |
Labeling reagent (amine-reactive) with green emission spectral properties (Abs 488 nm / EM 517 nm) after removal of acetate esters, intracellularly.
|
| M0093 |
Isethionyl acetimidate sodium salt hydrochloride |
Cell membrane cross-linking reagent. |
| M0128 |
Biocytin Hydrazide Hydrochloride |
A water-soluble, long-chained, biotin-containing hydrazide, used for the selective detection of glycoconjugates. Biocytin hydrazide is a selective label for sialic acids, galactose, and other sugars in glycoconjugates using avidin-biotin technology. |
| M0270 |
Monofluoresceinated Mannitol (MFM) |
MFM is a new product that has shown use in labeling of the interstitial (intercellular) spaces between neurons in vitro. Since D-mannitol is preferentially excluded by neuronal cell membranes, this derivative of mannitol has been used to measure the fluid area between neurons. |
| M0329 |
3-Dansylaminophenylboronic acid |
Fluorescent labeling agent for cis-diols (sugars) at high pH values on cell surfaces and in vitro for carbohydrate determination in glycoproteins and for HPLC analysis of sugars. The fluorescence emission of the Dansyl fluorophore is environmentally sensitive. M0329 is also known to be an active site inhibitor for lipases. |
| M0384 |
8-Azidoadenosine 5-Triphosphate, TEA salt |
Light tan solid. Useful in photoaffinity labelling of ATP binding proteins. Covalent labeling after exposure to UV light (e.g. UVS-11 or UVG-11 lamp). Not radiolabeled. |
| M0500 |
NBD-X, SE |
This sulfosuccinimidyl ester is a fluorescent dye used to label amines. See the references below for more details on use. |
| M0524 |
Pyrene 1-Sulfonyl Chloride |
A useful amine reactive reagent for labeling proteins and peptides. The fluorescence emission is environment dependent. |
| M0530 |
N-(di-Methyl Amino Naphthyl)-N-(para Carboxy Phenyl) hydrazide |
This NEW* long wavelength azo dye is a useful reagent (similar to DABCYL-succinimidyl ester) for labeling proteins or peptides through their amino-groups. The succinimidyl ester is reactive with amines and other nucleophiles for labeling studies of peptides and proteins. |
| M0532 |
N-(di-Methyl Amino Naphthyl)-N-(para Carboxy Phenyl) hydrazide, NHS ester |
This longer wavelength azo dye quencher is a useful reagent (similar to DABCYL-succinimidyl ester) for labeling proteins or peptides through their amino-groups. The succinimidyl ester is reactive with amines and other nucleophiles for labeling studies of peptides and proteins. |
| M0535 |
Pyrene 8-Hydroxy-1, 4, 6-trisulfonyl Chloride |
This pyrene derivative is useful for labeling amine groups of proteins, peptides or other compounds (drugs), and has unusual pH dependent fluorescence properties and pKa changes, after conjugation. 8-hydroxy-1,3,6-trisulfonate-pyrene (HPTS) is often called a "photoacid" because it can be transformed by light from a weak acid into a strong acid. |
| M0638 |
5-(Iodoacetamido)fluorescein |
Excellent thiol reactive fluorescein derivative for synthesis of fluorescently labeled organelles, proteins and peptides at or near their physiological pH values (pH 7) including enzymes (e.g. (Na, K)-ATPase) or organelles like nucleii and nuclear matrices. |
| M0783 |
Biotin-X, SE |
This reactive biotin derivative contains an aminohexanoyl spacer group (termed "X") between biotin and the binding site for proteins, nucleic acids, or other biological molecules. This spacer helps to reduce potential quenching effects of fluorophores attached to the analyte molecule, since biotin binds to "deep" binding sites in avidin or streptavidin. This extra linking arm makes it more accessible for binding, and improves a variety of assays employing biotin-avidin or biotin-streptavidin systems. This amine reactive (succinimidyl ester, SE) derivative can be readily coupled to primary or secondary amines of analyte compounds or proteins under mild conditions. Biotin is a commonly used hapten that can be detected by using its strong affinity for the proteins avidin (from egg white) or streptavidin. Many avidin or streptavidin conjugates are available that have been fluorescently labeled or are conjugated to an antibody or other detection reagent. This biotin labeling compound has been used in many such detection schemes for peptides, proteins, organelle components, monoclonal antibody analyses, oligonucleotide and microbead labelings. See references below for further information on the use of biotin labeling. |
| M0785 |
Biotin Succinimidyl Ester |
This activated biotin derivative can be readily coupled to primary or secondary amines of analyte compounds or proteins under mild conditions. Biotin is a commonly used hapten that can be detected by using its strong affinity for the proteins avidin (from egg white) or streptavidin. Many avidin or streptavidin conjugates are available that have been fluorescently labeled or are conjugated to an antibody or other detection reagent. This biotin labeling compound has been used in many such detection schemes for peptides, proteins, organelle components, monoclonal antibody analyses, oligonucleotide and microbead labelings. |
| M0955 |
MarkerGeneTM FITC Antibody Labeling Kit |
This NEW* kit provides reagents and methods to simply and quickly label antibodies or other proteins with the brilliant green dye fluorescein. |
| M0972 |
MarkerGeneTM TAMRA Antibody/Protein/Cell Labeling Kit |
This NEW* kit provides reagents and methods to simply and quickly label antibodies or other proteins with the amine-reactive dye 5(6)-carboxytetramethylrhodamine. |
| M0979 |
5(6)-Carboxyfluorescein-hexanoic acid, NHS ester |
This useful fluorescent amine-reactive probe has an added spacer to improve accessibility of the reactive group by nucelophiles in preparation of bioconjugates. |
| M0984 |
5(6)-Carboxyfluorescein, NHS ester |
Carboxyfluorescein succinimidyl ester is a useful fluorescent amine-reactive probe for synthesis of bioconjugates, yielding hydrolysis resistant derivatives which show excellent performance and stability at physiological pH values. |
| M1025 |
4-Hydrazino-7-nitrobenzofurazane (NBD Hydrazine) |
Fluorescent derivatization reagent useful for aliphatic aldehyde detection including reducing carbohydrates. |
| M1036 |
Fluorescein-5-thiosemicarbazide |
This cell impermeant fluorescent probe is useful for determining protein and peptide topology on the cell surface. |
| M1051 |
4-((4-(dimethylamino)phenyl)azo)benzoic acid, Succinimidyl ester (DABCYL-SE) |
This long wavelength azo dye is a useful reagent (similar to MANCYL-SE) for labeling proteins or peptides through their amino-groups. The succinimidyl ester is reactive with amines and other nucleophiles for labeling studies of peptides and proteins. |
| M1053 |
7-diethylaminocoumarin-3-carboxylic acid, Succinimidyl ester |
This amine reactive fluorescent probe is useful for labeling amino acids prior to resolution by capillary zone electrophoresis and for multicolor DNA and protein labeling experiments. |
| M1074 |
Eosin 5-isothiocyanate (EITC) |
Eosin 5-isothiocyanate can be used as a reversible inhibitor of the erythrocyte calcium pump as well as an effective phosphorescent probe in protein rotational studies. Eosin derivatives are also used in diaminobenzidine photo-oxidation processes and fluorescence resonance energy transfer (FRET). |
| M1076 |
10-Octadecylacridine orange bromide |
A useful fluorescent mitochondrial membrane probe used to analyze mitochondria by flow cytometry and measure changes in mitochondrial mass during apoptosis. |
| M1093 |
Ethidium Homodimer (EthD-1) |
Ethidium Homodimer is a high affinity fluorescent nucleic stain. It binds to both DNA and RNA in a sequence-independent manner and with a >30-fold fluorescence enhancement. The DNA binding of each Ethidium Hodimer covers four base pairs and is believed to occur by intercalation. Because the dye is highly positively charged, it cannot cross cell membranes to stain living cells. |
| M1111 |
2-Iminobiotin |
Reversible inhibitor of mouse inducible and rat neuronal nitric oxide synthase (iNOS/NOS II; nNOS/NOS I). Also useful for affinity purification of avidin on immobilized solid supports. |
| M1120 |
Sulforhodamine 101 sulfonyl chloride |
Reactive red fluorophore which couples covalently to amino groups on proteins and other compounds. Suitable for use with FITC in dual parameter flow cytometry studies. Equivalent to Texas RedTM. |
| M1124 |
Avidin Sulforhodamine 101 Conjugate (Texas RedTM equivalent) |
Fluorescein, Texas RedTM as well as TAMRA conjugates of Avidin and Streptavidin have become increasingly useful for DNA and RNA microarray analyses, as a method of detecting biotin-labeled antibodies, or in ELISA assays. Marker Gene's Texas RedTM Avidin is a highly fluorescent conjugate of Avidin and sulforhodamine 101. This red fluorescent protein excites at 595 nm with an emission maximum at 615 nm. Since the excitation and emission maxima are well separated from those of fluorescein, Texas RedTM Avidin can be employed with other fluorescein or coumarin conjugates to simultaneously localize two or three antigens in the same tissue section. Texas RedTM Avidin is ideal for flow cytometry applications using instruments equipped with dye lasers. The great affinity of avidin for D-biotin (Ka = 10-15/M) provides for a great number of applications in biochemistry (immunoassays, receptor and histochemical studies, bacteriophage inhibitions). This fluorescently labeled avidin conjugate combines stoichiometrically with biotin, having 4-biotin binding sites per avidin molecule. In addition, the Texas RedTM Avidin is a highly cationic glycoprotein that can selectively bind to a component in human and murine mast cell granules in fixed-cell preparations, and can thus be used to identify mast cells in normal and diseased human tissue without requiring a biotinylated probe. See also our Fluorescein Isothiocyanate (FITC) Avidin Conjugate, Product M1274.
|
| M1127 |
Dansyl-ß-lactoglobulin-A |
IEF-Marker for HPCE and for IEF-gels. Fluorescent IEF Marker pI 5.1 with EX 330nm and EM 415 nm in 0.1 M citrate buffer pH 5.1. |
| M1138 |
MarkerGeneTM Biotin-X Antibody/Protein Labeling Kit |
For efficient biotinylation of antibodies and other proteins.The kit contains biotin-X succinimidyl ester, which reacts with primary amines of proteins, antibodies or other biomolecules to form stable biotin conjugates. The long spacer between the biotin and the reactive group in biotin-X succinimidyl ester enhances the ability of the conjugated biotin to interact with avidin and streptavidin. Also included in the kit are a gel filtration spin-column to purify the biotinylated protein from excess biotin, reagents for quantitating the degree of biotinylation and a control. This kit contains sufficient reagents for at least five biotinylation reactions of 1–5 mg each. |
| M1197 |
3,3-dioctadecyloxacarbocyanine perchlorate (DiO; DiOC18(3)) |
A highly fluorescent membrane probe for labeling live and fixed cells in culture. Long-chain dialkylcarbocyanines are widely used as anterograde and retrograde neuronal tracers in living and fixed tissues and cells. |
| M1202 |
Eosin-5-thiouredylphenylboronic acid, triethylammonium salt |
M1202 is a new long-wavelength fluorescent labeling agent for cis-diols (sugars) at high pH values on cell surfaces and in vitro for carbohydrate determination in glycoproteins and for HPLC analysis of sugars. This labeling agent can be used in conjunction with other fluorophores, for multiplexed analysis, in fluorescence energy transfer (FRET) studies or in a high-throughput format (EX 520/ EM544). The fluorescence emission of the Eosin labeled compound can exhibit environment sensitive fluorescence emission properties. The eosin derivatives can also exhibit phosphorescence with an emission maximum at ~680 nm. See the references below for more information on use of M1202. |
| M1224 |
Rhodamine B octadecyl ester perchlorate |
Lipophilic energy transfer acceptor from lipophilic fluoresceins in fluorescence energy transfer (FRET) assays in cell fusion experiments. Also used as a probe for measuring potassium in fiber-optic sensors.
|
| M1227 |
8-Octadecyloxypyrene-1,3,6-trisulfonic acid trisodium salt |
Highly water soluble polar fluorescent lipid probe for the sensitive fluorimetric assay of cationic surfactants. |
| M1257 |
Berberine sulfate |
Berberine is useful as a fluorescent stain for heparin in several cell lines. It is an effector for MDR (multidrug resistance) efflux pumps and is potentiated by the MDR inhibitor 5'-methoxyhydnocarpin (5'-MHC). Treatment with berberine potentially results in the reduced accumulation of chemotherapeutic drugs. Berberine upregulates the expression of Pgp in hepatoma cells. |
| M1259 |
5(6)-Carboxynaphthofluorescein, N-succinimidyl ester |
Reactive long wavelength fluorophore for use in labeling proteins and other compounds. Reactive with terminal and internal (Lys) groups, at pH 8-9. The fluorescence properties are pH dependent.
See also our product M1248 for more information and uses. |
| M1269 |
1,1'-Dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine perchlorate |
Red fluorescent membrane probe for long term staining of live cells. Also used as in fibre optic sensors for detecting K+ using fluorescence energy transfer.
|
| M1274 |
Avidin Fluorescein Isothiocyanate (FITC) Conjugate |
Fluorescein, Texas RedTM as well as TAMRA conjugates of Avidin and Streptavidin have become increasingly useful for DNA and RNA microarray analyses, as a method of detecting biotin-labeled antibodies, or in ELISA assays. FITC Avidin is a highly fluorescent conjugate of Avidin and fluorescein isothiocyanate. This green fluorescent protein excites at 494 nm with an emission maximum at 518 nm. Since the excitation and emission maxima are well separated from those of other fluorophores such as Texas RedTM and coumarin, FITC Avidin can be employed with other conjugates to simultaneously localize two or three antigens in the same tissue section. FITC Avidin is ideal for flow cytometry applications using instruments equipped with dye lasers. The great affinity of avidin for D-biotin (Ka = 10-15/M) provides for a great number of applications in biochemistry (immunoassays, receptor and histochemical studies, bacteriophage inhibitions). This fluorescently labeled avidin conjugate combines stoichiometrically with biotin, having 4-biotin binding sites per avidin molecule. In addition, the FITC Avidin is a highly cationic glycoprotein that can selectively bind to a component in human and murine mast cell granules in fixed-cell preparations, and can thus be used to identify mast cells in normal and diseased human tissue without requiring a biotinylated probe. See also our Texas RedTM Avidin Conjugate, Product M1124.
|
| M1282 |
Ethidium Bromide |
Commonly used nucleic acid stain. Fluorescence of Ethidium Bromide increases 25-fold upon intercalation with double-stranded DNA and 21-fold upon intercalation with double-stranded-RNA, eliminating the need to destain background. |
| M1331 |
3,3'-Dipropylthiacarbocyanine iodide |
DiS-C3(3) is a membrane potential sensitive dye for monitoring changes in the cellular and mitochondrial membrane potential in vivo. This dye can exhibit fluorescence changes of up to 80% upon membrane hyperpolarization (where the inside of the cell becomes more negative). The fluorescence emission and excitation (EX = 556 nm; EM = 575nm in MeOH; EX = 520 nm, EM = 595nm in water) are in the red region of the visible spectrum, allowing co-staining with other viability stains. |
| M1356 |
MarkerGeneTM Biotin Detection and Quantitation Kit |
The MarkerGeneTM Biotin Detection and Quantitation Kit allows the measurement of the molar ratio of biotin to protein in biotin-protein conjugates, biotin-DNA molar ratios in biotinylated DNA samples, or quantitation of biotin concentrations in solution. The assay employs the sensitive chromogenic reagent 4'-hydroxyazobenzene-2-carboxylic acid (HABA) that exhibits a UV spectral absorbance at 500 nm when bound to avidin. Because biotin easily displaces HABA from HABA/Avidin complexes, the resulting decrease in absorption at 500 nm can be used to sensitively measure biotin concentrations. The kit can be used to determine biotin concentrations down to 1 uM concentrations. The assay can be performed in a microtiterplate format. |
