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Interest in lactosidase enzymes has increased, as it has been found to be important in detection of various enzyme deficient diseases such as ceramide lactoside liposis, Fabry's Disease and lactose intolerance. Lactosidase (lactase) is a hydrolytic enzyme (a ß-galactosidase) that acts on a number of lactosides, cutting either the internal linkage in lactose at the Gal(ß1-4)Glc linkage and producing glucosylated aglycones and D-galactose or removing the disaccharide in one step. Most cells also contain ß-glucosidase, that can be used to complete release of the fluorophore or chromogen used in common cell assay conditions.
| ID
| Name
| CAS
| Unit Size
| Price
| Bulk Price (5+) |
| M0554 |
4-Methylumbelliferyl β-D-lactoside |
84325-23-5 |
25 mg |
83.24 |
66.59 |
| Fluorescent substrate for β-lactosidase or galactosidase and glucosidase activities; releases 4-methylumbelliferone (EX 385 nm, EM 502 nm, e = 16K) upon enzyme action. The fluorescence is optimal at pH values above 8-9. |
|
| M0553 |
2-Chloro-4-nitrophenyl-β-D-lactoside |
120583-41-7 |
25 mg |
106.95 |
85.56 |
| Chromogenic substrate for lactosidase activity. The released product, 2-chloro-4-nitrophenol (CNP) is usually monitored spectrophotometrically at 405 nm. The rate of formation of 2-chloro-4-nitrophenol is proportional
to the lactosidase activity in the sample. The pKa of CNP is approximately 5.5. |
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