L-Leucine 7-amido-4-methyl coumarin hydrochloride (Leu-AMC.HCl)

Product ID: M1060

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Sensitive fluorogenic substrate for leucine aminopeptidase and aminopeptidase M detection which releases the bright blue fluorescent dye, 7-amino-4-methylcoumarin M1059 upon enzyme activity with emission 440nm upon at excitation 380nm.

This fluorogenic enzyme substrate L-Leucine-4-methylcoumaryl-7-amide - Leu-AMC is widely used as a high sensitive substrate for aminopeptidase M and for leucine aminopeptidase. Leu-MCA (Methyl-Cumarinyl Amide) is another synonym for Leu-AMC. The sensitivity of this enzyme substrate using fluorescence detection is greatly increased over other methods such as 4-Methoxy-naphthylamide (pNA) assays.

Technical Data
SKU M1060
CAS Number 62480-44-8
Unit Size 25mg
Alternative Names L-Leucine-4-methyl-7-coumarinylamide Hydrochloride
Absorption 325nm
Emission Wavelength 395
Detection Method Fluorescence
Molecular Formula C₁₆H₂₀N₂O₃.HCl
Molecular Weight 324.8
Soluble In DMSO, MeOH
Storage Conditions 2-8C, Desiccated
Notes Abs = 380nm/Em = 440nm (after leucine aminopeptidase cleavage)

References and Citations


  • "Profiles of bacterial community composition and metabolic potential through a plug-flow bioreactor fed with lake water." Riemann L, Sondergaard M. Journal of Plankton Research. 26(8): 973-978 (2004).
  • "Comparison of different peptidase substrates for evaluation of microbial quality of aerobically stored meats." Stepaniak L. J Food Prot. 63(10): 1447-1449 (2000).
  • "Visual detection of peptidase activity using fluorogenic substrates in a microtiter plate assay." Irvine GB, Ennis M, Williams CH. Anal Biochem. 185(2): 304-307 (1990).
  • "Subcellular localisation of leucine aminopeptidase in human polymorphonuclear leukocytes." Smith GP, MacGregor RR, Peters TJ. Biochim Biophys Acta. 728(2): 222-7 (1983).
  • "Localization of leucine aminopeptidase and vitamin B-12 binding protein in rabbit peripheral blood polymorphonuclear leukocytes." Smith GP, MacGregor RR, Peters TJ. Biochim Biophys Acta. 719(3): 532-538 (1982).
  • "Direct photometric or fluorometric assay of proteinases using substrates containing 7-amino-4-trifluoromethylcoumarin." Smith RE, Bissell ER, Mitchell AR, Pearson KW. Thromb Res. 17(3-4): 393-402 (1980).
  • "A novel fluorometric ultramicro determination of serum leucine aminopeptidase using a coumarine derivative." Saifuku K, Sekine T, Namihisa T, Takahashi T, Kanaoka Y. Clin Chim Acta. 84(1-2): 85-91 (1978).
  • "A new fluorogenic substrate for aminopeptidase." Kanaoka Y, Takahashi T, Nakayama H. Chem Pharm Bull (Tokyo). 25(2): 362-3 (1977).
  • Methods of Enzymatic Analysis Vol. I, 26, Academic Press: New York (1975).
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