Lipases are a family of enzymes including cholesterol esterases and glycerol ester hydrolases that release fatty acids from triacylglycerols in a site-specific manner. Most lipases have optimum activity for the primary ester groups of triglycerides, while some lipases remove fatty acyl groups from either the C-1 or C-3 acyl positions. The substrate is typically not a single molecule, but a non-aqueous phase of aggregated lipid. Fuorescent triglyceride substrates also have applications in other areas including micelle and membrane labeling, fluorescence energy transfer experiments, and membrane fluidity studies.
This kit allows fast and easy measurement of lipase activity in vitro, in cell preparations or in vivo using the fluorescent triglyceride substrate 1,2-dioleoyl-3-(pyren-1-yl)decanoyl-rac-glycerol M0258. Learn More