MarkerGene™ Live Cell Luciferase Assay Kit

Product ID: M0626



Unit SizePriceQuantity 
1kit
$131.20
  • Buy 5 for $104.96 each and save 20%

Availability: In stock


Description

This kit contains the all of the reagents including the primary substrate of the firefly luciferase light producing system, D-luciferin, as well as the lysis and luciferase enzyme assay buffers.

Firefly luciferase (EC 1.13.12.7) is the enzyme used in chemiluminescent reactions, producing light by the oxidation of D-luciferin with oxygen and adenosine triphosphate (ATP). Luciferase is used in numerous biochemical assays, where the gene is either cloned into cells or tissues as a reporter or marker gene. The MarkerGene™ Live Cell Luciferase Assay Kit kit will perform 1000 luciferase assays using a microtiterplate format (100 uL reaction volume, 0.1 mM final reaction concentration). The synthetic beetle luciferin substrate contained in the kit is also available separately as a lyophilized powder, D-Luciferin M0237. Consult the Product information sheet (above) for more details on the assay protocol.

Technical Data
SKU M0626
Unit Size 1kit
Detection Method Luminescence

References and Citations

Citations:

  • Thomas M, Lu JJ, Ge Q, Zhang C, Chen J, Klibanov AM. (2005) "Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung." Proceedings of the National Academy of Sciences of the United States of America, 102(16), 5679–5684.
  • Ge Q, Filip L, Bai A, Nguyen T, Eisen HN, Chen J. (2004) "Inhibition of influenza virus production in virus-infected mice by RNA interference." Proc Natl Acad Sci U S A. 101(23):8676-81.
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References:

  • Gildea JJ, et al. (2000) "Transmembrane motility assay of transiently transfected cells by fluorescent cell counting and luciferase measurement." Biotechniques 29: 81.
  • Van Leeuwen W, et al. (2000) "The use of the luciferase reporter system in plant gene expression studies." Plant Mol. Biol. Rep. 18:143a.
  • Matthews BF, et al. (1995) "Reporter genes and transient assays for plants." Methods Mol. Biol. 55:147.
  • Jha PK, et al. (1990)"Firefly luciferase, synthesized to very high levels in caterpillars infected with a recombinant baculovirus, can also be used as an effective reporter enzyme in vivo." FEBS Lett. 274: 23.
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