Luciferase

A variety of species have evolved the ability to emit light biochemically. Among those that have found the most use in molecular biology applications are the North American firefly photinus pyralis, the marine copepod Gaussia princeps and the bacterium Vibrio harveyi. The firefly luciferase gene (luc) has been cloned into a variety of species, becoming a popular marker gene for plants, bacteria and mammalian cells. Measurement of transciptional regulation by coupling the expression of a reporter gene is widely used to monitor a variety of physiological events including the action of specific receptor elements on gene expression, signal transduction, expression of transcription factors, protein-protein interactions, viral infection and processing. The firefly luciferase gene encodes a functional protein immediately upon transcription, and an assay for the resulting light production is highly sensitive (very low background), rapid, and reliable, making luciferase a popular choice for reporter cloning experiments. These assays are also well suited to automation and high-throughput applications. The substrate, coelenterazine is common to a number of marine bioluminescent reactions, including those from Renilla, Gaussia, Aequorea and Watesenia. In some of these reactions it is utilized as a simple substrate being catalytically turned over in the bioluminescent reaction, while in others, such as in the photoprotein systems of Mneiopsis, it is incorporated as part of the photoprotein. The substrate h-coelenterate luciferin is a coelenterazine luciferin analog wherein one of the phenolic groups is replaced with a proton. This substrate exhibits a high recharging efficiency and even higher light output.

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  1. D-Luciferin

    D-Luciferin

    $93.56

    Primary substrate of the firefly luciferase light producing system. Learn More
  2. Phenethyl-1-thio-β-D-glucopyranosiduronic acid (PETGU)

    Phenethyl-1-thio-β-D-glucopyranosiduronic acid (PETGU)

    $41.10

    β-Glucuronidase inhibitor similar to PETG. Learn More
  3. 6-Amino-D-luciferin

    6-Amino-D-luciferin

    $164.65

    Amino analog of the D-luciferin substrate for measuring firefly luciferase activity. Learn More
  4. MarkerGene™ Live Cell Luciferase Assay Kit

    MarkerGene™ Live Cell Luciferase Assay Kit

    $131.20

    This kit contains the all of the reagents including the primary substrate of the firefly luciferase light producing system, D-luciferin, as well as the lysis and luciferase enzyme assay buffers. Learn More
  5. Coelenterazine (native form)

    Coelenterazine (native form)

    $54.43

    Coelenterazine is the native form of the luminescent enzyme substrate for apoaequorin, gaussia and renilla luciferases. This substrate is also a component of our convenient assay kit, MarkerGene™ Gaussia Luciferase Cellular Assay Kit, Product M1193, for quickly measuring the luciferase activity in mammalian cell culture. Learn More
  6. 6-FMOC-Amino-D-luciferin

    6-FMOC-Amino-D-luciferin

    $230.48

    Amino analog of the D-luciferin substrate for firefly luciferase activity. It can be used in automated peptide synthesis for incorporation of amino-luciferin into synthetic peptides. Learn More
  7. MarkerGene™ Chemiluminescent lacZ β-Galactosidase Detection Kit

    MarkerGene™ Chemiluminescent lacZ β-Galactosidase Detection Kit

    $295.72

    Allows for quantitative measure of β-galactosidase enzyme activity in mammalian, yeast, and bacterial cells through use of a chemiluminescent substrate. Learn More
  8. D-Luciferin, ethyl ester

    D-Luciferin, ethyl ester

    $78.40

    Membrane permeable analog of the primary substrate for the firefly luciferase light producing system with 30% higher light intensity. Learn More

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