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Enzyme Substrates and Kits for Luciferase

A variety of species have evolved the ability to emit light biochemically. Among those that have found the most use in molecular biology applications are the North American firefly photinus pyralis, the marine copepod Gaussia princeps and the bacterium Vibrio harveyi.

The firefly luciferase gene (luc) has been cloned into a variety of species, becoming a popular marker gene for plants, becteria and mammalian cells. Measurement of transciptional regulation by coupling the expression of a reporter gene is widely used to monitor a variety of physiological events including the action of specific receptor elements on gene expression, signal transduction, expression of transcription factors, protein-protein interactions, viral infection and processing. The firefly luciferase gene encodes a functional protein immediately upon transcription, and an assay for the resulting light production is highly sensitive (very low background), rapid, and reliable, making luciferase a popular choice for reporter cloning experiments. These assays are also well suited to automation and high-throughput applications.

The substrate, coelenterazine is common to a number of marine bioluminescent reactions, including those from Renilla, Gaussia, Aequorea and Watesenia. In some of these reactions it is utilized as a simple substrate being catalytically turned over in the bioluminescent reaction, while in others, such as in the photoprotein systems of Mneiopsis, it is incorporated as part of the photoprotein. The substrate h-coelenterate luciferin is a coelenterazine luciferin analog wherein one of the phenolic groups is replaced with a proton. This substrate exhibits a high recharging efficiency and even higher light output.

Now also available our newest RubyGlowLuciferase kits, click here for more information.

  ID   Name   CAS Unit Size Price Bulk Price (5+)
M1576   RubyGlowTM Luminescent Cytotoxicity Assay Kit 1 kit 110.00
Most commercially available luciferase kits use green light emitting (562 nm) firefly analogs. The RubyGlowTM luminescent assay kits produce a red light (EM 619 nm) for measurement of cell number, cell proliferation, growth, viability or cytotoxicity based upon the generation of ATP in metabolically active cells. This unique feature enables the possibility of multiplexing. The new kit components involve no radioisotopes or toxic materials and are environmentally safe. Each kit provides enough reagents and a detailed protocol sufficient for 100 reactions in 96-well microplate format.
M1575   RubyGlowTM Luminescent Cell Viability Assay Kit 1 kit 110.00
Most commercially available luciferase kits use green light emitting (562 nm) firefly analogs. The RubyGlowTM luminescent assay kits produce a red light (EM 619 nm) for measurement of cell number, cell proliferation, growth, viability or cytotoxicity based upon the generation of ATP in metabolically active cells. This unique feature enables the possibility of multiplexing. The new kit components involve no radioisotopes or toxic materials and are environmentally safe. Each kit provides enough reagents and a detailed protocol sufficient for 100 reactions in 96-well microplate format.
M1574   RubyGlowTM Luminescent Cell Proliferation Assay Kit 1 kit 110.00
Most commercially available luciferase kits use green light emitting (562 nm) firefly analogs. The RubyGlowTM luminescent assay kits produce a red light (EM 619 nm) for measurement of cell number, cell proliferation, growth, viability or cytotoxicity based upon the generation of ATP in metabolically active cells. This unique feature enables the possibility of multiplexing. The new kit components involve no radioisotopes or toxic materials and are environmentally safe. Each kit provides enough reagents and a detailed protocol sufficient for 100 reactions in 96-well microplate format.
M1395   pDC99 Mammalian Luciferase Expression Vector N/A 20 μg 250.95 250.95
High copy number eukaryotic vector, pDC99 expresses a mutant luciferase gene under the control of the Simian virus 40Early Core (SV40) promoter. This vector encodes a mutant enzyme that catalyzes the production of long wavelength (red) light (Em:605) from D-luciferin, M0237.
M1394   pDC57 Mammalian Luciferase Expression Vector N/A 20 ug 250.95 250.95
High copy number eukaryotic vector, pDC57, expresses a mutant luciferase gene under the control of the cytomegalovirus immediate early gene (CMV IE) promoter. This vector encodes a mutant enzyme that catalyzes the production of long wavelength (red) light (Em:605) from D-luciferin, M0237.
M1307   pCMVLuxB Mammalian LuxB Expression Vector N/A 20 ug 117.81 117.81
High copy number eukaryotic vector, pCMVLuxB, expresses the beta subunit of luciferase encoded by the bacterium Vibrio harveyi. When paired with pCMVLuxA M1306, luminescence can be produced in mammalian cell systems.
M1306   pCMVLuxA Mammalian LuxA Expression Vector N/A 20 ug 117.81 117.81
High copy number eukaryotic vector, pCMVLuxA, expresses the alpha subunit of luciferase encoded by the bacterium Vibrio harveyi. When paired with pCMVLuxB M1307, luminescence can be produced in mammalian cell systems.
M0906   D-Luciferin, ethyl ester 135251-85-3 10 mg 60.80 48.64
Membrane permeable analog of the primary substrate for the firefly luciferase light producing system with 30% higher light intensity.
M0236   D-Luciferin, 6'-methyl ether 3022-11-5 5 mg 100.25 80.20
Inhibitor of the firefly luciferase light producing system. Useful in chemiluminescent dual assay systems with both luciferase and cytochrome P450 enzymes.
M1087   D-Luciferin-6-O-β-D-galactopyranoside 131474-38-9 5 mg 157.89 126.31
Analog of D-Luciferin contains a β-galactoside attached at the 6-O-position, and thus is not a substrate for the firefly luciferase enzyme until the galactose is removed e.g., by β-galactosidase activity. As such it represents an ultrasensitive substrate for chemiluminescent measurement of galactosidase activity in homogeneous assays, or in cell lysate samples when the enzyme luciferase is added.
M0237   D-Luciferin 2591-17-5 5 mg 76.00 60.80
Primary substrate of the firefly luciferase light producing system.
M0626   MarkerGene™ Live Cell Luciferase Assay Kit 1 kit 106.58 85.26
Contains synthetic D-luciferin, the primary substrate of the firefly luciferase light producing system, as well as a luciferase assay buffer
M1193   MarkerGene™ Gaussia Luciferase Cellular Assay Kit 1 kit 71.61 57.29
Gaussia luciferase is a new reporter gene isolated from the marine copepod Gaussia princeps, and can be expressed in mammalian cells using commercially available reporter plasmids. This luciferase, which does not require ATP, catalyzes the oxidation of the substrate (M0739) in a reaction that emits light (at 470 nm), and has considerable advantages over other reporter genes.
M0802   6-FMOC-Amino-D-luciferin 578705-74-2 5 mg 187.23 149.78
Amino analog of D-luciferin substrate for firefly luciferase activity. Useful in automated peptide synthesis for incorporation of amino-luciferin into synthetic peptides.
M0739   Coelenterazine (native form) 55779-48-1 50 μl* 44.21 35.36
The native form of the luminescent enzyme substrate for apoaequorin, gaussia and renilla luciferases. This substrate is also a component of our convenient assay kit, MarkerGene™ Gaussia Luciferase Cellular Assay Kit, Product M1193, for quickly measuring the luciferase activity in mammalian cell culture.
M0352   6-Amino-D-luciferin 118969-27-0 5 mg 133.75 107.00
Amino analog of D-luciferin substrate for firefly luciferase activity.
M1796   pFHluc Mammalian Luciferase Expression Vector with Cloning Site 20μg 250.00
High copy number eukaryotic vector, pFHluc, expresses a mutant luciferase gene under the control of the cytomegalovirus immediate early gene (CMV IE) promoter. Contains a multiple cloning site with 11 unique restriction sites. This vector encodes a mutant enzyme that catalyzes the production of long wavelength (red) light (Em:619) from D-luciferin, M0237.

©2012 Marker Gene Technologies, Inc. Published by Marker Gene Technologies, Inc., The University of Oregon Riverfront Research Park, 1850 Millrace Drive, Eugene, Oregon 97403-1992 USA. All rights reserved. For information on the use or copying of the material contained in this document, please contact us at techservice@markergene.com.