High Copy Number Expression Vectors for Luciferase (lux, luc).

A variety of species have evolved the ability to emit light biochemically. The luciferases from these various species have been cloned into a variety of species, becoming popular marker genes for plants, becteria and mammalian cells. Marker Gene provides a variety of high copy number expression vectors and is constantly developing new vectors and systems. Feel free to call our technical assistance staff for more information about these or other molecular biology products for your research.

Product ID Product Name Description
M1306 pCMVLuxA Mammalian LuxA Expression Vector  This high copy number eukaryotic vector, pCMVLuxA expresses the alpha subunit of luciferase encoded by the bacterium Vibrio harveyi. Bacterial luciferase is a heterodimeric protein, requiring an alpha and beta subunit for functional enzymatic activity. When paired with pCMVLuxB (Product M1307.), luminescence can be produced in mammalian cell systems. The LuxAB system can be used to monitor expression of two cloned genes using one reporter. Expression is under the control of the cytomegalovirus immediate early gene (CMV IE) promoter. LuxA expression is enhanced by the featured Kozak sequence, Splice Donor/Acceptor site (SV40 SD/SA) and SV40 late polyadenylylation signal. pCMVLuxA expression vector also contains also contains the β-lactamase gene, which acts as a selection marker (100ug/mL ampicillin resistance) in E. coli host. In addition, the LuxA gene can be excised using the flanking NotI sites to allow the insertion of other genes to be expressed under the same regulatory elements in mammalian cells.  
M1307 pCMVLuxB Mammalian LuxB Expression Vector  This high copy number eukaryotic vector, pCMVLuxB expresses the beta subunit of luciferase encoded by the bacterium Vibrio harveyi. Bacterial luciferase is a heterodimeric protein, requiring an alpha and beta subunit for functional enzymatic activity. When paired with pCMVLuxA (Product M1306.), luminescence can be produced in mammalian cell systems. The LuxAB system can be used to monitor expression of two cloned genes using one reporter. Expression is under the control of the cytomegalovirus immediate early gene (CMV IE) promoter. LuxB expression is enhanced by the featured Kozak sequence, Splice Donor/Acceptor site (SV40 SD/SA) and SV40 late polyadenylylation signal. pCMVLuxB expression vector also contains also contains the β-lactamase gene, which acts as a selection marker (100ug/mL ampicillin resistance) in E. coli host. In addition, the LuxB gene can be excised using the flanking NotI sites to allow the insertion of other genes to be expressed under the same regulatory elements in mammalian cells.  
     
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