Product ID: M1087

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Ultrasensitive substrate for chemiluminescent measurement of β-galactosidase activity in homogeneous assays, or in cell lysate samples when the enzyme luciferase is added.

This analog of D-Luciferin contains a β-galactoside attached at the 6-O-position, and thus is not a substrate for the firefly luciferase enzyme until the galactose is removed e.g., by β-galactosidase activity. When combined with firefly luciferase in coupled assays, the combined double substrate (Luc-Gal) has proven to have detection limits for β-galactosidase activity as low as 3 x 10(-19) mol of enzyme per assay. It has also found utility in detection of engineered bacteria in vivo.

Technical Data
SKU M1087
CAS Number 131474-38-9
Unit Size 5mg
Alternative Names D-Luciferin-6-O-β-D-galactoside
Detection Method Luminescence
Molecular Formula C₁₇H₁₈N₂O₈S₂
Molecular Weight 442.47
Soluble In H2O
Storage Conditions -20C, Desiccated

References and Citations


  • "Homogeneous enzyme immunoassay modified for application to luminescence-based biosensors." Yang,Y., Janatova, J., Andrade, J.D Anal. Biochem 33: 102-107 (2005).
  • "A new ultrasensitive bioluminogenic enzyme substrate for beta-galactosidase"· Geiger R., Schneider E., Wallenfels K., Miska W. Biol. Chem. Hoppe-Seyler 373(12): 1187-91 (1992).
  • "Bioluminescent assay of b-galactosidase using D-luciferin-o-b-galactoside." · Ugarova, N. N.; Voznyi, Ya. V.; Kutuzova, G. D.; Dementeva, E. I. Biolumin. Chemilumin. Proc. Int. Symp., 6th : 511-14 (1991).
  • "A new homogeneous enzyme immunoassay using recombinant enzyme fragments" · Khanna, P.L. Dworschack R.T., Manning W.B., Harris J.D. Clin. Chim. Acta 33: 231-39 (1989).
  • "CEDIA, a new homogeneous immunoassay system." · Henderson, D.R. Friedman, S.B. Harris, J.D. Manning, W.B. Zoccoli, M.A. Clin.Chem. 32: 1637-1641 (1986).
  • Geiger R., Schneider E., Wallenfels K., Miska W., (1992) "A new ultrasensitive bioluminogenic enzyme substrate for beta-galactosidase." Biol. Chem. Hoppe Seyler. 373(12):1187-91.
  • Tatsumi, H., Masuda, I., Kasai, K., Sano, A., "A method for detecting Escherichia coli group bacteria." Jpn. Kokai Tokkyo Koho (1999), 4 pp. JP 11332593
  • Masuda-Nishimura, I., Fukuda, S., Sano, A., Kasai, K., Tatsumi, H., (2000), "Development of a rapid positive/absent test for coliforms using sensitive bioluminescence assay." Letters in Applied Microbiology 30(2): 130-135.
  • Fukuda, S.; Murakami, S.; Tatsumi, H. Recombinant firefly luciferase and their application for the detection of microorganisms. Bioluminescence & Chemiluminescence, Proceedings of the International Symposium, 11th, Pacific Grove, CA, United States, Sept. 6-10, 2000 (2001), Meeting Date 2000, 285-288.
  • Yang, X., Janatova, J., Andrade, J.D. (2005),"Homogeneous enzyme immunoassay modified for application to luminescence-based biosensors." Analytical Biochemistry 336(1), 102-107.
  • Wehrman, T.S., von Degenfeld, G., Krutzik, P.O., Nolan, G.P., Blau, H.M. (2006), "Luminescent imaging of b-galactosidase activity in living subjects using sequential reporter-enzyme luminescence." Nature Methods 3(4):295-301.
  • Sano, A., Kasai, Koichi; Tatsumi, Hiroki; Masuda, Ikuko; Tokutake, Shoichi. (2000), "Preparation of D-Luciferin-O-b-D-glucuronide derivative, and its applications to measuring b-glucuronidase activity and detecting Escherichia coli. " Jpn. Kokai Tokkyo Koho 14 pp.
  • Miska, W., Monsees, T., Franken, D. R., Henkel, R.,(1997) "Evaluation of the bioluminescence-enhanced zona binding assay." Bioluminescence and Chemiluminescence: Molecular Reporting with Photons, Proceedings of the International Symposium on Bioluminescence and Chemiluminescence, 9th, Woods Hole, Mass., Oct. 4-8, 1996 Meeting Date 1996, 315-318.
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