Product ID: M0237

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Primary substrate of the firefly luciferase light producing system.

D-Luciferin, also called firefly luciferin, is the compound used by many Lampyridae species as the primary substrate for light emission with the enzyme luciferase (EC producing the characteristic yellow light emission from fireflies. Since the luciferase enzyme is now widely used in biochemical assays, this substrate is highly useful for a variety of ultrasensitive chemiluminescent methods. The biochemistry of the enzyme reaction requires adenosine triphosphate (ATP) and therefore can also be used to measure ATP levels, providing a HTS method of monitoring, cell number, cell health and viability. See also our various kits for measuring luciferase using this highly purified substrate

Technical Data
SKU M0237
CAS Number 2591-17-5
Unit Size 5mg
Alternative Names 4,5-dihydro-2[6-hydroxy-2-benzthiazolyl]-4-thiazole carboxylic acid
Detection Method Luminescence
Molecular Formula C₁₁H₈N₂O₃S₂
Molecular Weight 280.33
Storage Conditions -20C, Desiccated
Notes Free acid

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References and Citations


  • Aldrich MB, Wang X, Hart A, Kwon S, Sampath L, Marshall MV, Sevick-Muraca EM. (2011) "Assessment of free dye in solutions of dual-labeled antibody conjugates for in vivo molecular imaging." Mol Imaging Biol. 13(1):32-42.
  • Chandran SS, Williams SA, Denmeade SR. (2009) "Extended-release PEG-luciferin allows for long-term imaging of firefly luciferase activity in vivo." Luminescence. 24(1):35-8.
  • Sampath L, Kwon S, Ke S, Wang W, Schiff R, Mawad ME, Sevick-Muraca EM. (2007) "Dual-labeled trastuzumab-based imaging agent for the detection of human epidermal growth factor receptor 2 overexpression in breast cancer." J Nucl Med 48(9):1501-10.
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  • Gildea JJ, Harding MA, Gulding KM, Theodorescu D. (2000) "Transmembrane motility assay of transiently transfected cells by fluorescent cell counting and luciferase measurement." Biotechniques 29(1): 81-86.
  • Van Leeuwen W, et al. (2000) "The use of the luciferase reporter system for in planta gene expression studies." Plant Mol. Biol. Rep. 18: 143a.
  • Matthews BF, Saunders JA, Gebhardt JS, Lin JJ, Koehler SM. (1995) "Reporter genes and transient assays for plants." Methods Mol. Biol. 55: 147-162.
  • Bronstein I, Fortin J, Stanley PE, Stewart GS, Kricka LJ. (1994) "Chemiluminescent and bioluminescent reporter gene assays." Anal Biochem 219(2): 169-181.
  • Jha PK, Nakhai B, Sridhar P, Talwar GP, Hasnain SE. (1990) "Firefly luciferase, synthesized to very high levels in caterpillars infected with a recombinant baculovirus, can also be used as an effective reporter enzyme in vivo." FEBS Lett. 274(1-2): 23-26.
  • Ow DW, et al. (1986) "Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants." Science 234: 856.
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