MarkerGene™ LysoLive™ Lysosomal Acid Lipase Assay Kit
Product ID: M2887
This kit utilizes the sensitive substrate LipaGreen™ to monitor acid lipase activity in the lysosomes of live cells by flow cytometry.
The MarkerGene™ LysoLive™ Lysosomal Acid Lipase Assay Kit utilizes a sensitive substrate for lysosomal acid lipase (LAL), an enzyme indicated in several diseases such as Lysosomal Acid Lipase Deficiency (LALD). The substrate only becomes fluorescent upon cleavage by the enzyme meaning lysosomes with low acid lipase activity will have reduced staining in a manner proportional to the enzyme activity levels found in the cell.
|Unit Size||1 kit|
|Storage Conditions||-20C, Protect From Light|
- Harlan FK, Lusk JS, Mohr BM, Guzikowski AP, Batchelor RH, Jiang Y, et al. (2016) "Fluorogenic Substrates for Visualizing Acidic Organelle Enzyme Activities." PLoS ONE 11(5): e0156312. doi:10.1371/journal.pone.0156312
- Gokce S. (2016) “The Key Clinical Manifestations of Lysosomal Acid Lipase Deficiency.” J Pediatr Gastroenterol Nutr. 63(3):e39.
- Karageorgos LE, Isaac EL, Brooks DA, Ravenscroft EM, Davey R, Hopwood JJ, Meikle PJ. (1997) “Lysosomal biogenesis in lysosomal storage disorders.” Experimental Cell Research 234, 85-97.
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