MarkerGene™ LysoLive™ Lysosomal β-Galactosidase Assay Kit
Product ID: M2776
This kit utilizes the sensitive substrate GalGreen™ to monitor beta-galactosidase activity in the lysosomes of live cells.
The MarkerGene™ LysoLive™ Lysosomal beta-Galactosidase Assay Kit utilizes a sensitive substrate for lysosomal beta-galactosidase (GALC), an enzyme indicated in several diseases such as Krabbe disease and Morquio Syndrome. The substrate only becomes fluorescent upon cleavage by the enzyme meaning lysosomes with low beta-Galactosidase activity will have reduced staining in a manner proportional to the enzyme activity levels found in the cell.
|Unit Size||1 kit|
- Harlan FK, Lusk JS, Mohr BM, Guzikowski AP, Batchelor RH, Jiang Y, et al. (2016) "Fluorogenic Substrates for Visualizing Acidic Organelle Enzyme Activities." PLoS ONE 11(5): e0156312. doi:10.1371/journal.pone.0156312
- Suzuki K, Suzuki Y (1970) "Globoid cell leucodystrophy (Krabbe’s disease): Deficiency of Lysosomal Galactocerebroside -galactosidase" Proceedings of the National Academy of Sciences 66(2):302–309.
- Kolodny EH, Raghavan S, Krivit W. (1991) “Late-onset Krabbe disease (globoid cell leukodystrophy): clinical and biochemical features of 15 cases.” Dev Neurosci. 13(4-5):232-9.
Karageorgos LE, Isaac EL, Brooks DA, Ravenscroft EM, Davey R, Hopwood JJ, Meikle PJ (1997) “Lysosomal biogenesis in lysosomal storage disorders.” Experimental Cell Research 234, 85-97.
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