MarkerGene™ LysoLive™ Lysosomal β-Glucosidase Assay Kit
Product ID: M2775
This kit utilizes the sensitive substrate GlucGreen™ to monitor beta-glucosidase activity in the lysosomes of live cells.
Lysosomal beta-glucosidase enzyme is currently indicated to be involved in diseases such as Gaucher and Parkinson’s diseases. MarkerGene™ LysoLive™ Lysosomal beta-Glucosidase Assay Kit utilizes a substrate which only becomes fluorescent upon cleavage by the enzyme meaning lysosomes with low beta-Glucosidase (GBA) activity will exhibit reduced staining in a manner proportional to the enzyme activity levels in the cell.
|Unit Size||1 kit|
|Storage Conditions||-20C, Protect From Light|
- Harlan FK, Lusk JS, Mohr BM, Guzikowski AP, Batchelor RH, Jiang Y, et al. (2016) "Fluorogenic Substrates for Visualizing Acidic Organelle Enzyme Activities." PLoS ONE 11(5): e0156312. doi:10.1371/journal.pone.0156312
- Westbroek W, Gustafson AM, Sidransky E. (2011) “Exploring the link between glucocerebrosidase mutations and parkinsonism.” Trends Mol Med. 17(9):485-493.
- Sidransky E. (2005) “Gaucher disease and parkinsonism.” Mol. Genet. Metab. 84:302-304.
- Sidransky E (2004) "Gaucher disease: complexity in a “simple” disorder" Molecular genetics and metabolism 83(1-2):6-15.
- Karageorgos LE, Isaac EL, Brooks DA, Ravenscroft EM, Davey R, Hopwood JJ, Meikle PJ (1997) “Lysosomal biogenesis in lysosomal storage disorders.” Experimental Cell Research 234, 85-97.
- Neudorfer O, Giladi N, Elstein D, Abrahamov A (1996) "Occurrence of Parkinson’s syndrome in type 1 Gaucher disease." Qjm 691-694.
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