MarkerGene™ LysoLive™ Lysosomal Metabolic Health Assay Kit
Product ID: M1910
The MarkerGene™LysoLive™ Lysosomal Metabolic Health Assay Kit utilizes the sensitive substrate EsterGreen™ to stain only actively metabolizing lysosomes.
Lysosomes are acidic cytoplasmic organelles that are present in all nucleated mammalian cells. Lysosomes have been found to be involved in a variety of cellular processes including repair of the plasma membrane, defense against pathogens, cholesterol homeostasis, bone remodeling, metabolism, apoptosis, and cell signaling. Defects in lysosomal enzyme activity have been associated with a variety of diseases including Parkinson’s, Tay-Sachs, Sandhoff, Krabbe, Wolman, and Gaucher syndromes. Marker Gene has developed lysosomal staining compounds that are useful for labeling lysosomes in a live-cell format and are capable of monitoring lysosomal metabolic activity.
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- Harlan FK, Lusk JS, Mohr BM, Guzikowski AP, Batchelor RH, Jiang Y, et al. (2016) "Fluorogenic Substrates for Visualizing Acidic Organelle Enzyme Activities." PLoS ONE 11(5): e0156312. doi:10.1371/journal.pone.0156312
- Carpenter AE,Jones TR, Lamprecht MR, Clarke C, Kang IH, Friman O, Guertin DA, Chang JH, Lindquist RA, Moffat J, Golland P, Sabatini DM (2006) “CellProfiler: image analysis software for identifying and quantifying cell phenotypes.” Genome Biology 7:R100.
- Michihara A, Toda K, Kubo T, Fujiwara Y, Akasaki K, Tsuji H. (2005) “Disruptive effectof chloroquine on lysosomes in cultured rat hepatocytes.” Biol Pharm Bull. 28(6):947-51.
- Karageorgos LE, Isaac EL, Brooks DA, Ravenscroft EM, Davey R, Hopwood JJ, Meikle PJ (1997) “Lysosomal Biogenesis in Lysosomal Storage Disorders” Experimental Cell Research 234, 85-97.
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