LysoLive™pH-Sensor Green

Product ID: M1790



Unit SizePriceQuantity 
3 x 50uL
$61.60

Availability: In stock


Description

Supplied as a 1 mM solution in anhydrous DMSO, ready for use.

LysoLive™pH-Sensor Green is a new membrane permeant live cell stain that is targeted in the cell to acidic organelles, including lysosomes, autophagocytic vescicles and peroxisomes. Our LysoLive™pH-Sensor Green probe is virtually non-fluorescent at physiological pH values in the cytosol, but becomes much more fluorescent when it accumulates in acidic environments. The pKa of LysoLive™pH-Sensor Green is similar to other commercially available lysosomal probes such as LysoSensor™ Green DND-189 at about 5.4. Our new probe exhibits improved loading and staining characteristics to existing pH sensors. It is supplied in DMSO that can be diluted directly into media or PBS to obtain working solution.

Technical Data
SKU M1790
Unit Size 3 x 50uL
Absorption 450
Emission Wavelength 520
Extinction 77
Detection Method Fluorescence
Molecular Weight 514.65
Soluble In DMSO
Storage Conditions F, D, L

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References and Citations

References:

  • Neuhaus EM, Soldati T. (2000) "A myosin I is involved in membrane recycling from early endosomes." J Cell Biol 150:1013-1026.
  • Brazill DT, Caprette DR, Myler HA, Hatton RD, Ammann RR, Lindsey DF, Brock DA, Gomer RH. (2000) "A protein containing a serine-rich domain with vesicle fusing properties mediates cell cycle-dependent cytosolic pH regulation." J Biol Chem 275:19231-19240.
  • Guttenberger M. (2000) "Arbuscules of vesicular-arbuscular mycorrhizal fungi inhabit an acidic compartment within plant roots." Planta 211:299-304.
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