M0952-pSV40Beta Mammalian lacZ Expression Vector Restriction Sites

AfeI       (AGC'GCT) [Eco47III,Aor51HI,FunI]

Cuts 1 time.

Cuts at position 2672.

 

AhdI       (GACnn_n'nnGTC) [Eam1105I,AspEI,DriI,EclHKI]

Cuts 1 time.

Cuts at position 5663.

 

AvrII      (C'CTAG_G) [AspA2I,BlnI,XmaJI]

Cuts 1 time.

Cuts at position 381.

 

BaeI       (ACnnnnGTAyCnnnnnnn_nnnnn')

Cuts 1 time.

Cuts at position 1061.

 

BaeI       (GrTACnnnnGTnnnnnnnnnn_nnnnn')

Cuts 1 time.

Cuts at position 1094.

 

BbeI       (G_GCGC'C) [NarI,EgeI,EheI,KasI,Mly113I,SfoI]

Cuts 1 time.

Cuts at position 6889.

 

BclI       (T'GATC_A) [FbaI,Ksp22I]

[dam methylated]

Cuts 1 time.

Cuts at position [2183].

 

BglII      (A'GATC_T)

Cuts 1 time.

Cuts at position 517.

 

BlpI       (GC'TnA_GC) [Bpu1102I,Bsp1720I,CelII]

Cuts 1 time.

Cuts at position 3850.

 

BplI       (GAGnnnnnCTCnnnnnnnn_nnnnn')

Cuts 1 time.

Cuts at position 348.

 

Bpu10I     (CC'TnA_GC)

Cuts 1 time.

Cuts at position 1342.

 

BsaI       (GGTCTCn'nnnn_) [Eco31I,Bso31I,BspTNI]

Cuts 1 time.

Cuts at position 5724.

 

BsaBI      (GATnn'nnATC) [Bse8I,BseJI,MamI]

[dam methylated]

Cuts 1 time.

Cuts at position [2168].

 

BsiWI      (C'GTAC_G) [Pfl23II,PspLI,SunI]

Cuts 1 time.

Cuts at position 3608.

 

BssHII     (G'CGCG_C) [BsePI,PauI]

Cuts 1 time.

Cuts at position 2335.

 

BstZ17I    (GTA'TAC) [BssNAI,Bst1107I]

Cuts 1 time.

Cuts at position 3602.

 

Bsu36I     (CC'TnA_GG) [AxyI,Bse21I,Eco81I]

Cuts 1 time.

Cuts at position 1061.

 

ClaI       (AT'CG_AT) [BanIII,Bsa29I,BseCI,Bsp106I,BspDI,BspXI,Bsu15I,BsuTUI,ZhoI]

[dam methylated]

Cuts 1 time.

Cuts at position 1661.

 

DraIII     (CAC_nnn'GTG) [AdeI]

Cuts 1 time.

Cuts at position 2027.

 

EcoICRI    (GAG'CTC) [SacI,Ecl136II,Psp124BI,SstI]

Cuts 1 time.

Cuts at position 2775.

 

EcoRI      (G'AATT_C) [FunII]

Cuts 1 time.

Cuts at position 1.

 

EcoRV      (GAT'ATC) [Eco32I]

Cuts 1 time.

Cuts at position 1950.

 

HindIII    (A'AGCT_T)

Cuts 2 times.

 

KasI       (G'GCGC_C) [NarI,BbeI,EgeI,EheI,Mly113I,SfoI]

Cuts 1 time.

Cuts at position 6885.

 

MfeI       (C'AATT_G) [MunI]

Cuts 1 time.

Cuts at position 4327.

 

NarI       (GG'CG_CC) [BbeI,EgeI,EheI,KasI,Mly113I,SfoI]

Cuts 1 time.

Cuts at position 6886.

 

NcoI       (C'CATG_G) [Bsp19I]

Cuts 1 time.

Cuts at position 288.

 

NotI       (GC'GGCC_GC) [CciNI]

Cuts 2 times.

 

PpuMI      (rG'GwC_Cy) [PpuXI,Psp5II,PspPPI]

[dcm methylated]

Cuts 1 time.

Cuts at position 583.

 

PsiI       (TTA'TAA)

Cuts 1 time.

Cuts at position 4298.

 

PstI       (C_TGCA'G) [BspMAI]

Cuts 1 time.

Cuts at position 4403.

 

SacI       (G_AGCT'C) [Ecl136II,EcoICRI,Psp124BI,SstI]

Cuts 1 time.

Cuts at position 2777.

 

SalI       (G'TCGA_C)

Cuts 1 time.

Cuts at position 4393.

 

SapI       (GCTCTTCn'nnn_)

Cuts 1 time.

Cuts at position 4654.

 

SbfI       (CC_TGCA'GG) [Sse8387I,SdaI]

Cuts 1 time.

Cuts at position 4403.

 

ScaI       (AGT'ACT) [ZrmI]

Cuts 1 time.

Cuts at position 6143.

 

SexAI      (A'CCwGG_T) [MabI]

[dcm methylated]

Cuts 1 time.

Cuts at position [148].

 

SfiI       (GGCCn_nnn'nGGCC)

[dcm methylated]

Cuts 1 time.

Cuts at position 334.

 

SfoI       (GGC'GCC) [NarI,BbeI,EgeI,EheI,KasI,Mly113I]

Cuts 1 time.

Cuts at position 6887.

 

SmaI       (CCC'GGG) [Cfr9I,PspAI,XmaI,XmaCI]

Cuts 1 time.

Cuts at position 713.

 

XbaI       (T'CTAG_A)

[dam methylated]

Cuts 1 time.

Cuts at position 4387.

 

XhoI       (C'TCGA_G) [BssHI,PaeR7I,Sfr274I,SlaI,TliI]

Cuts 1 time.

Cuts at position 521.

 

XmaI       (C'CCGG_G) [SmaI,Cfr9I,PspAI,XmaCI]

Cuts 1 time.

Cuts at position 711.

 

XmnI       (GAAnn'nnTTC) [Asp700I,MroXI,PdmI]

Cuts 1 time.

Cuts at position 6262.