M1015-pCMVBeta Mammalian lacZco Expression Vector Restriction Sites

AarI       (CACCTGCnnnn'nnnn_)

Cuts 1 time.

Cuts at position 3626.

 

AccI       (GT'mk_AC) [FblI,XmiI]

Cuts 2 times.

 

AclI       (AA'CG_TT) [Psp1406I]

Cuts 2 times.

 

AcuI       (CTGAAGnnnnnnnnnnnnnn_nn') [Eco57I]

Cuts 2 times.

 

AfeI       (AGC'GCT) [Eco47III,Aor51HI,FunI]

Cuts 2 times.

 

AhdI       (GACnn_n'nnGTC) [Eam1105I,AspEI,DriI,EclHKI]

Cuts 2 times.

 

AleI       (CACnn'nnGTG) [OliI]

Cuts 2 times.

 

AlfI       (GCAnnnnnnTGCnnnnnnnnnn_nn')

Cuts 1 time.

Cuts at position 1756.

 

ApaI       (G_GGCC'C) [Bsp120I,PspOMI]

[dcm methylated]

Cuts 1 time.

Cuts at position [3400].

 

ApoI       (r'AATT_y) [AcsI,XapI]

Cuts 2 times.

 

BanII      (G_rGCy'C) [Eco24I,EcoT38I,FriOI]

Cuts 2 times.

 

BbsI       (GAAGACnn'nnnn_) [BpiI,BpuAI,BstV2I]

Cuts 1 time.

Cuts at position 600.

 

BciVI      (GTATCCnnnnn_n') [BfuI]

Cuts 2 times.

 

BglII      (A'GATC_T)

Cuts 1 time.

Cuts at position 1358.

 

BplI       (GAGnnnnnCTCnnnnnnnn_nnnnn')

Cuts 2 times.

 

BsaBI      (GATnn'nnATC) [Bse8I,BseJI,MamI]

[dam methylated]

Cuts 2 times.

 

BseRI      (GAGGAGnnnnnnnn_nn')

Cuts 2 times.

 

BsmI       (GAATG_Cn') [BsaMI,Mva1269I,PctI]

Cuts 2 times.

 

BspEI      (T'CCGG_A) [AccIII,BseAI,Bsp13I,Kpn2I,MroI]

[dam methylated]

Cuts 1 time.

Cuts at position 617.

 

BsrDI      (GCAATG_nn') [Bse3DI,BseMI]

Cuts 2 times.

 

BsrGI      (T'GTAC_A) [Bsp1407I,BstAUI,SspBI]

Cuts 1 time.

Cuts at position 3470.

 

BssHII     (G'CGCG_C) [BsePI,PauI]

Cuts 1 time.

Cuts at position 1227.

 

BstAPI     (GCAn_nnn'nTGC)

Cuts 1 time.

Cuts at position 6437.

 

BstEII     (G'GTnAC_C) [BstPI,Eco91I,EcoO65I,PspEI]

Cuts 1 time.

Cuts at position 2607.

 

BstXI      (CCAn_nnnn'nTGG)

Cuts 1 time.

Cuts at position 3297.

 

BtgZI      (GCGATGnnnnnnnnnn'nnnn_)

Cuts 1 time.

Cuts at position 300.

 

ClaI       (AT'CG_AT) [BanIII,Bsa29I,BseCI,Bsp106I,BspDI,BspXI,Bsu15I,BsuTUI,ZhoI]

[dam methylated]

Cuts 1 time.

Cuts at position 656.

 

DraIII     (CAC_nnn'GTG) [AdeI]

Cuts 1 time.

Cuts at position 1898.

 

DrdI       (GACnn_nn'nnGTC) [AasI,DseDI]

Cuts 2 times.

 

EcoICRI    (GAG'CTC) [SacI,Ecl136II,Psp124BI,SstI]

Cuts 1 time.

Cuts at position 532.

 

EcoRI      (G'AATT_C) [FunII]

Cuts 1 time.

Cuts at position 1.

 

FseI       (GG_CCGG'CC)

Cuts 1 time.

Cuts at position 2831.

 

FspAI      (rTGC'GCAy)

Cuts 1 time.

Cuts at position 1702.

 

HindIII    (A'AGCT_T)

Cuts 2 times.

 

HpaI       (GTT'AAC) [KspAI]

Cuts 1 time.

Cuts at position 3920.

 

MfeI       (C'AATT_G) [MunI]

Cuts 1 time.

Cuts at position 3929.

 

MscI       (TGG'CCA) [BalI,MlsI,MluNI,Msp20I]

[dcm methylated]

Cuts 2 times.

 

NdeI       (CA'TA_TG) [FauNDI]

Cuts 2 times.

 

NotI       (GC'GGCC_GC) [CciNI]

Cuts 1 time.

Cuts at position 3776.

 

PfoI       (T'CCnGG_A)

Cuts 1 time.

Cuts at position 6298.

 

PmlI       (CAC'GTG) [PmaCI,AcvI,BbrPI,Eco72I,PspCI]

Cuts 1 time.

Cuts at position 1374.

 

PpuMI      (rG'GwC_Cy) [PpuXI,Psp5II,PspPPI]

[dcm methylated]

Cuts 1 time.

Cuts at position 3450.

 

PsiI       (TTA'TAA)

Cuts 1 time.

Cuts at position 3900.

 

PspOMI     (G'GGCC_C) [ApaI,Bsp120I]

Cuts 1 time.

Cuts at position 3396.

 

PspXI      (vC'TCGA_Gb)

Cuts 1 time.

Cuts at position 640.

 

PvuI       (CG_AT'CG) [BspCI,Ple19I]

Cuts 2 times.

 

SacI       (G_AGCT'C) [Ecl136II,EcoICRI,Psp124BI,SstI]

Cuts 1 time.

Cuts at position 534.

 

SacII      (CC_GC'GG) [Cfr42I,KspI,Sfr303I,SgrBI]

Cuts 2 times.

 

SalI       (G'TCGA_C)

Cuts 2 times.

 

SapI       (GCTCTTCn'nnn_)

Cuts 1 time.

Cuts at position 4256.

 

ScaI       (AGT'ACT) [ZrmI]

Cuts 2 times.

 

SfiI       (GGCCn_nnn'nGGCC)

[dcm methylated]

Cuts 1 time.

Cuts at position [3367].

 

SgrAI      (Cr'CCGG_yG)

Cuts 1 time.

Cuts at position 1231.

 

SnaBI      (TAC'GTA) [BstSNI,Eco105I]

Cuts 1 time.

Cuts at position 306.

 

SphI       (G_CATG'C) [BbuI,PaeI,SpaHI]

Cuts 2 times.

 

SspI       (AAT'ATT)

Cuts 1 time.

Cuts at position 6069.

 

StuI       (AGG'CCT) [AatI,Eco147I,PceI,SseBI]

[dcm methylated]

Cuts 2 times.

 

TfiI       (G'AwT_C) [PfeI]

Cuts 2 times.

 

Tth111I    (GACn'n_nGTC) [AspI,PflFI,PsyI,TelI]

Cuts 1 time.

Cuts at position 1467.

 

XcmI       (CCAnnnn_n'nnnnTGG)

Cuts 2 times.

 

XhoI       (C'TCGA_G) [BssHI,PaeR7I,Sfr274I,SlaI,TliI]

Cuts 1 time.

Cuts at position 640.

 

XmnI       (GAAnn'nnTTC) [Asp700I,MroXI,PdmI]

Cuts 2 times.