M1016-pSV40Beta Mammalian lacZco Expression Vector Restriction Sites

AarI       (CACCTGCnnnn'nnnn_)

Cuts 1 time.

Cuts at position 3507.

 

AatII      (G_ACGT'C) [ZraI]

Cuts 1 time.

Cuts at position 6068.

 

AccI       (GT'mk_AC) [FblI,XmiI]

Cuts 2 times.

 

AclI       (AA'CG_TT) [Psp1406I]

Cuts 2 times.

 

AcuI       (CTGAAGnnnnnnnnnnnnnn_nn') [Eco57I]

Cuts 2 times.

 

AfeI       (AGC'GCT) [Eco47III,Aor51HI,FunI]

Cuts 2 times.

 

AhdI       (GACnn_n'nnGTC) [Eam1105I,AspEI,DriI,EclHKI]

Cuts 2 times.

 

AleI       (CACnn'nnGTG) [OliI]

Cuts 2 times.

 

ApaI       (G_GGCC'C) [Bsp120I,PspOMI]

[dcm methylated]

Cuts 1 time.

Cuts at position [3281].

 

ApoI       (r'AATT_y) [AcsI,XapI]

Cuts 2 times.

 

AvrII      (C'CTAG_G) [AspA2I,BlnI,XmaJI]

Cuts 1 time.

Cuts at position 381.

 

BanII      (G_rGCy'C) [Eco24I,EcoT38I,FriOI]

Cuts 1 time.

Cuts at position 3281.

 

BciVI      (GTATCCnnnnn_n') [BfuI]

Cuts 2 times.

 

BfrBI      (ATG'CAT) [EcoT22I,Mph1103I,NsiI,Zsp2I]

Cuts 2 times.

 

BglII      (A'GATC_T)

Cuts 2 times.

 

BsaBI      (GATnn'nnATC) [Bse8I,BseJI,MamI]

[dam methylated]

Cuts 2 times.

 

BsmI       (GAATG_Cn') [BsaMI,Mva1269I,PctI]

Cuts 2 times.

 

BsmBI      (CGTCTCn'nnnn_) [Esp3I]

Cuts 2 times.

 

BsrDI      (GCAATG_nn') [Bse3DI,BseMI]

Cuts 2 times.

 

BsrGI      (T'GTAC_A) [Bsp1407I,BstAUI,SspBI]

Cuts 1 time.

Cuts at position 3351.

 

BssHII     (G'CGCG_C) [BsePI,PauI]

Cuts 1 time.

Cuts at position 1108.

 

BstEII     (G'GTnAC_C) [BstPI,Eco91I,EcoO65I,PspEI]

Cuts 1 time.

Cuts at position 2488.

 

BstXI      (CCAn_nnnn'nTGG)

Cuts 1 time.

Cuts at position 3178.

 

ClaI       (AT'CG_AT) [BanIII,Bsa29I,BseCI,Bsp106I,BspDI,BspXI,Bsu15I,BsuTUI,ZhoI]

[dam methylated]

Cuts 1 time.

Cuts at position 537.

 

DraIII     (CAC_nnn'GTG) [AdeI]

Cuts 1 time.

Cuts at position 1779.

 

DrdI       (GACnn_nn'nnGTC) [AasI,DseDI]

Cuts 2 times.

 

EcoRI      (G'AATT_C) [FunII]

Cuts 1 time.

Cuts at position 1.

 

FalI       (AAGnnnnnCTTnnnnnnnn_nnnnn')

Cuts 1 time.

Cuts at position 432.

 

FseI       (GG_CCGG'CC)

Cuts 1 time.

Cuts at position 2712.

 

FspAI      (rTGC'GCAy)

Cuts 1 time.

Cuts at position 1583.

 

HpaI       (GTT'AAC) [KspAI]

Cuts 1 time.

Cuts at position 3801.

 

MfeI       (C'AATT_G) [MunI]

Cuts 1 time.

Cuts at position 3810.

 

MscI       (TGG'CCA) [BalI,MlsI,MluNI,Msp20I]

[dcm methylated]

Cuts 2 times.

 

NdeI       (CA'TA_TG) [FauNDI]

Cuts 1 time.

Cuts at position 6317.

 

NotI       (GC'GGCC_GC) [CciNI]

Cuts 1 time.

Cuts at position 3657.

 

NsiI       (A_TGCA'T) [BfrBI,EcoT22I,Mph1103I,Zsp2I]

Cuts 2 times.

 

PfoI       (T'CCnGG_A)

Cuts 1 time.

Cuts at position 6179.

 

PmlI       (CAC'GTG) [PmaCI,AcvI,BbrPI,Eco72I,PspCI]

Cuts 1 time.

Cuts at position 1255.

 

PpuMI      (rG'GwC_Cy) [PpuXI,Psp5II,PspPPI]

[dcm methylated]

Cuts 1 time.

Cuts at position 3331.

 

PsiI       (TTA'TAA)

Cuts 1 time.

Cuts at position 3781.

 

PspOMI     (G'GGCC_C) [ApaI,Bsp120I]

Cuts 1 time.

Cuts at position 3277.

 

PvuI       (CG_AT'CG) [BspCI,Ple19I]

Cuts 2 times.

 

SacII      (CC_GC'GG) [Cfr42I,KspI,Sfr303I,SgrBI]

Cuts 2 times.

 

SalI       (G'TCGA_C)

Cuts 2 times.

 

SapI       (GCTCTTCn'nnn_)

Cuts 1 time.

Cuts at position 4137.

 

SbfI       (CC_TGCA'GG) [Sse8387I,SdaI]

Cuts 2 times.

 

ScaI       (AGT'ACT) [ZrmI]

Cuts 2 times.

 

SexAI      (A'CCwGG_T) [MabI]

[dcm methylated]

Cuts 1 time.

Cuts at position [148].

 

SfiI       (GGCCn_nnn'nGGCC)

[dcm methylated]

Cuts 2 times.

 

SgrAI      (Cr'CCGG_yG)

Cuts 1 time.

Cuts at position 1112.

 

SspI       (AAT'ATT)

Cuts 1 time.

Cuts at position 5950.

 

TfiI       (G'AwT_C) [PfeI]

Cuts 2 times.

 

Tth111I    (GACn'n_nGTC) [AspI,PflFI,PsyI,TelI]

Cuts 1 time.

Cuts at position 1348.

 

XbaI       (T'CTAG_A)

[dam methylated]

Cuts 2 times.

 

XcmI       (CCAnnnn_n'nnnnTGG)

Cuts 2 times.

 

XhoI       (C'TCGA_G) [BssHI,PaeR7I,Sfr274I,SlaI,TliI]

Cuts 1 time.

Cuts at position 521.

 

XmnI       (GAAnn'nnTTC) [Asp700I,MroXI,PdmI]

Cuts 2 times.

 

ZraI       (GAC'GTC) [AatII]

Cuts 1 time.

Cuts at position 6066.